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COPS5 sirna

COPS5 siRNA (Mouse)

Gene Names
Cops5; CSN5; Jab1; Sgn5; Mov34; AI303502
Reactivity
Mouse
Applications
RNA Interference (RNAi)
Purity
> 97%
Synonyms
COPS5; COPS5 siRNA (Mouse); CSN5; JAB1; KIC2; COP9 signalosome complex subunit 5; SGN5; Signalosome subunit 5; Jun activation domain-binding protein 1; Kip1 C-terminus-interacting protein 2; COPS5 sirna
Ordering
For Research Use Only!
Host
Synthetic
Reactivity
Mouse
Specificity
COPS5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Sequence Length
317
Applicable Applications for COPS5 sirna
RNA Interference (RNAi)
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COPS5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Related Product Information for COPS5 sirna
siRNA to inhibit COPS5 expression using RNA interference

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,549 Da
NCBI Official Full Name
COP9 signalosome complex subunit 5 isoform 2
NCBI Official Synonym Full Names
COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana)
NCBI Official Symbol
Cops5
NCBI Official Synonym Symbols
CSN5; Jab1; Sgn5; Mov34; AI303502
NCBI Protein Information
COP9 signalosome complex subunit 5
UniProt Protein Name
COP9 signalosome complex subunit 5
UniProt Gene Name
Cops5
UniProt Synonym Gene Names
Csn5; Jab1; Kic2; SGN5; Signalosome subunit 5
UniProt Entry Name
CSN5_MOUSE

Uniprot Description

COPS5: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B). The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, p27Kip1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Belongs to the peptidase M67A family. CSN5 subfamily.

Protein type: Nuclear receptor co-regulator; EC 3.4.-.-; Translation; Protease; Transcription, coactivator/corepressor; Adaptor/scaffold

Cellular Component: nucleoplasm; signalosome; synaptic vesicle; transcription factor complex; cytoplasm; synapse; cytoplasmic vesicle; cell junction; nucleus

Molecular Function: peptidase activity; protein binding; metallopeptidase activity; hydrolase activity; metal ion binding; transcription coactivator activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: protein deneddylation; cullin deneddylation; protein deubiquitination; regulation of transcription, DNA-dependent; regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; proteolysis; regulation of JNK cascade

Research Articles on COPS5

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Product Notes

The COPS5 cops5 (Catalog #AAA8229731) is a siRNA produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The COPS5 siRNA (Mouse) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's COPS5 can be used in a range of immunoassay formats including, but not limited to, RNA Interference (RNAi). Researchers should empirically determine the suitability of the COPS5 cops5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COPS5, siRNA" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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