Human COP9 signalosome complex subunit 5 (COPS5) ELISA Kit | COPS5 elisa kit

Human COP9 signalosome complex subunit 5 (COPS5) ELISA Kit

Cat. Num. AAA7222529

• Gene Names: COPS5; CSN5; JAB1; SGN5; MOV-34
• Reactivity: Human
• Synonyms: COP9 signalosome complex subunit 5 (COPS5); Human COP9 signalosome complex subunit 5 (COPS5) ELISA Kit; COPS5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COPS5. No significant cross-reactivity or interference between COPS5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COPS5 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COPS5 elisa kit

Intended Uses: This COPS5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human COPS5. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: COPS5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COPS5 antibody and an COPS5-HRP conjugate. The assay sample and buffer are incubated together with COPS5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COPS5 concentration since COPS5 from samples and COPS5-HRP conjugate compete for the anti-COPS5 antibody binding site. Since the number of sites is limited, as more sites are occupied by COPS5 from the sample, fewer sites are left to bind COPS5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COPS5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for COPS5 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 38027923
• NCBI GeneID: 10987
• NCBI Accession #: NP_006828.2
• NCBI GenBank Nucleotide #: NM_006837.2
• Molecular Weight: 37,579 Da
• NCBI Official Full Name: COP9 signalosome complex subunit 5
• NCBI Official Synonym Full Names: COP9 signalosome subunit 5
• NCBI Official Symbol: COPS5
• NCBI Official Synonym Symbols: CSN5; JAB1; SGN5; MOV-34
• NCBI Protein Information: COP9 signalosome complex subunit 5; 38 kDa Mov34 homolog; signalosome subunit 5; jun activation domain-binding protein 1; COP9 constitutive photomorphogenic homolog subunit 5
• UniProt Protein Name: COP9 signalosome complex subunit 5
• UniProt Gene Name: COPS5
• UniProt Synonym Gene Names: CSN5; JAB1; SGN5; Signalosome subunit 5
• UniProt Entry Name: CSN5_HUMAN

NCBI Description

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

COPS5: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B). The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, p27Kip1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Belongs to the peptidase M67A family. CSN5 subfamily.

Protein type: Nuclear receptor co-regulator; Adaptor/scaffold; Transcription, coactivator/corepressor; EC 3.4.-.-; Protease; Translation

Chromosomal Location of Human Ortholog: 8q13.1

Cellular Component: nucleoplasm; eukaryotic translation initiation factor 3 complex; signalosome; synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; nucleus; cell junction

Molecular Function: protein binding; metallopeptidase activity; translation initiation factor activity; metal ion binding; transcription coactivator activity; ubiquitin-specific protease activity

Biological Process: transcription from RNA polymerase II promoter; protein deneddylation; cullin deneddylation; protein deubiquitination; translation; regulation of cell cycle; translational initiation; positive regulation of transcription from RNA polymerase II promoter; proteolysis; regulation of JNK cascade