Rabbit anti-Human, Rat NOS I Polyclonal Antibody

NOS I, phosphorylated (Ser1417) (Neuronal Nitric Oxide Synthase, bNOS, nNOS, NOS-1)

Cat. Num. AAA629394

• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NOS I; Polyclonal Antibody; phosphorylated (Ser1417) (Neuronal Nitric Oxide Synthase; bNOS; nNOS; NOS-1); Anti -NOS I; anti-NOS I antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human phosphorylated nNOS/NOS I, Mr 170kD. Species Cross-reactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.6, 0.175M sodium chloride, 0.07% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-NOS I antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500 detects affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK in vitro. Lambda phosphatase treated and untreated affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK was resolved by electrophoresis, transferred to PVDF and probed with N5350-02 (1:500). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa surrounding Ser1417 of human neuronal Nitric Oxide Synthase, with an N-terminal cysteine added for KLH conjugation purposes, where pS is phosphorylated serine corresponding to the AMPK phosphorylation site.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: Lamdba phosphatase treated (Lane 1) and untreated (Lane 2) affinity purified bacterial expressed rat nNOS/NOS I that had been phosphorylated by purified AMPK was probed with MBS629394 (0.5ug/ml).)

Related Product Information for anti-NOS I antibody

Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II).

Product Categories/Family for anti-NOS I antibody

Antibodies; Abs to Nitric Oxide

NCBI and Uniprot Product Information

• NCBI GI #: 295410244
• NCBI Official Full Name: NOS