COL9A1 sirna

COL9A1 siRNA (Mouse)

Cat. Num. AAA8240578

• Gene Names: Col9a1; Col9a-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL9A1; COL9A1 siRNA (Mouse); Collagen alpha-1(IX) chain; COL9A1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COL9A1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 921

• Applicable Applications for COL9A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COL9A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL9A1 sirna

siRNA to inhibit COL9A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226693380
• NCBI GeneID: 12839
• NCBI Accession #: NP_031766.3
• NCBI GenBank Nucleotide #: NM_007740.3
• UniProt Accession #: Q05722; Q61269; Q61270; Q61433; Q61940
• Molecular Weight: 65,493 Da
• NCBI Official Full Name: collagen alpha-1(IX) chain isoform 1
• NCBI Official Synonym Full Names: collagen, type IX, alpha 1
• NCBI Official Symbol: Col9a1
• NCBI Official Synonym Symbols: Col9a-1
• NCBI Protein Information: collagen alpha-1(IX) chain
• UniProt Protein Name: Collagen alpha-1(IX) chain
• Protein Family: Collagen
• UniProt Gene Name: Col9a1
• UniProt Entry Name: CO9A1_MOUSE

Uniprot Description

COL9A1: Structural component of hyaline cartilage and vitreous of the eye. Defects in COL9A1 are the cause of multiple epiphyseal dysplasia type 6 (EDM6). A generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. Radiological examination of the skeleton shows delayed, irregular mineralization of the epiphyseal ossification centers and of the centers of the carpal and tarsal bones. Multiple epiphyseal dysplasia is broadly categorized into the more severe Fairbank and the milder Ribbing types. The Fairbank type is characterized by shortness of stature, short and stubby fingers, small epiphyses in several joints, including the knee, ankle, hand, and hip. The Ribbing type is confined predominantly to the hip joints and is characterized by hands that are normal and stature that is normal or near-normal. Defects in COL9A1 are the cause of Stickler syndrome type 4 (STL4). An autosomal recessive form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; collagen; extracellular region

Molecular Function: metal ion binding

Biological Process: cartilage development; tissue homeostasis