CHRM3 sirna

CHRM3 siRNA (Mouse)

Cat. Num. AAA8223454

• Gene Names: Chrm3; M3; M3R; Chrm-3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRM3; CHRM3 siRNA (Mouse); CHRM-3; Muscarinic acetylcholine receptor M3; Mm3 mAChR; CHRM3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHRM3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 589

• Applicable Applications for CHRM3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHRM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRM3 sirna

siRNA to inhibit CHRM3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15149489
• NCBI GeneID: 12671
• NCBI Accession #: NP_150372.1
• NCBI GenBank Nucleotide #: NM_033269.4
• UniProt Accession #: Q9ERZ3; Q64055
• Molecular Weight: 66,212 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M3
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 3, cardiac
• NCBI Official Symbol: Chrm3
• NCBI Official Synonym Symbols: M3; M3R; Chrm-3
• NCBI Protein Information: muscarinic acetylcholine receptor M3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: Chrm3
• UniProt Synonym Gene Names: Chrm-3
• UniProt Entry Name: ACM3_MOUSE

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Cellular Component: asymmetric synapse; postsynaptic membrane; membrane; integral to plasma membrane; dendrite; integral to membrane; plasma membrane; synapse; nerve terminal; cell junction

Molecular Function: G-protein coupled receptor activity; signal transducer activity; acetylcholine receptor activity; drug binding; acetylcholine binding; G-protein coupled acetylcholine receptor activity

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; acetylcholine receptor signaling, muscarinic pathway; smooth muscle contraction; regulation of vasoconstriction; digestion; regulation of vascular smooth muscle contraction; positive regulation of smooth muscle contraction; signal transduction; saliva secretion; muscarinic acetylcholine receptor, phospholipase C activating pathway; synaptic transmission, cholinergic