CFLAR sirna

CFLAR siRNA (Mouse)

Cat. Num. AAA8228151

• Gene Names: Cflar; Cash; Flip; MRIT; CLARP; FLAME; Casper; Gm9845; c-Flip; FLAME-1; I-FLICE; 2310024N18Rik; A430105C05Rik; ENSMUSG00000072980
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CFLAR; CFLAR siRNA (Mouse); CASH; CASP8 and FADD-like apoptosis regulator; Caspase homolog; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; CFLAR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CFLAR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 481

• Applicable Applications for CFLAR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CFLAR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CFLAR sirna

siRNA to inhibit CFLAR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 576067795
• NCBI GeneID: 12633
• NCBI Accession #: NP_001276633.1
• NCBI GenBank Nucleotide #: NM_001289704.2
• UniProt Accession #: O35732; O35707; O35733; D3Z0W6
• Molecular Weight: 24,815 Da
• NCBI Official Full Name: CASP8 and FADD-like apoptosis regulator isoform 1
• NCBI Official Synonym Full Names: CASP8 and FADD-like apoptosis regulator
• NCBI Official Symbol: Cflar
• NCBI Official Synonym Symbols: Cash; Flip; MRIT; CLARP; FLAME; Casper; Gm9845; c-Flip; FLAME-1; I-FLICE; 2310024N18Rik; A430105C05Rik; ENSMUSG00000072980
• NCBI Protein Information: CASP8 and FADD-like apoptosis regulator
• UniProt Protein Name: CASP8 and FADD-like apoptosis regulator
• Protein Family: CASP8 and FADD-like apoptosis regulator
• UniProt Gene Name: Cflar
• UniProt Synonym Gene Names: Cash; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT
• UniProt Entry Name: CFLAR_MOUSE

Uniprot Description

CFLAR: Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. TNFRSF6 stimulation triggers recruitment to the death- inducing signaling complex (DISC) formed by TNFRSF6, FADD and caspase-8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with caspase-10, caspase-3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with HBV protein X. Repressed by IL2/interleukin-2 after TCR stimulation, during progression to the S phase of the cell cycle. Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. Belongs to the peptidase C14A family. 14 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Cellular Component: cytoplasm

Molecular Function: protease activator activity; protein heterodimerization activity

Biological Process: caspase activation; negative regulation of caspase activity; proteolysis; negative regulation of apoptosis