CDK5 sirna

CDK5 siRNA (Mouse)

Cat. Num. AAA8218278

• Gene Names: Cdk5; Crk6; AW048668
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK5; CDK5 siRNA (Mouse); CDKN5; CRK6; Cyclin-dependent kinase 5; CR6 protein kinase; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit; CDK5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDK5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 292

• Applicable Applications for CDK5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDK5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK5 sirna

siRNA to inhibit CDK5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6680908
• NCBI GeneID: 12568
• NCBI Accession #: NP_031694.1
• NCBI GenBank Nucleotide #: NM_007668.3
• UniProt Accession #: P49615
• Molecular Weight: 33,288 Da
• NCBI Official Full Name: cyclin-dependent kinase 5
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5
• NCBI Official Symbol: Cdk5
• NCBI Official Synonym Symbols: Crk6; AW048668
• NCBI Protein Information: cyclin-dependent-like kinase 5
• UniProt Protein Name: Cyclin-dependent-like kinase 5
• Protein Family: CDK5RAP1-like protein
• UniProt Gene Name: Cdk5
• UniProt Synonym Gene Names: Cdkn5; Crk6; CRK6; TPKII catalytic subunit
• UniProt Entry Name: CDK5_MOUSE

Uniprot Description

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: Protein kinase, CMGC; Kinase, protein; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; CMGC group; CDK family; CDK/CDK5 subfamily; CDK5 subfamily

Cellular Component: cyclin-dependent protein kinase 5 activator complex; cell; postsynaptic density; dendrite; cytosol; postsynaptic membrane; cell projection; cytoskeleton; growth cone; cell soma; membrane; lamellipodium; axon; cytoplasm; plasma membrane; nucleolus; synapse; intracellular; neuromuscular junction; nucleus; cell junction; filopodium

Molecular Function: acetylcholine receptor activator activity; ephrin receptor binding; p53 binding; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; ErbB-2 class receptor binding; protein serine/threonine kinase activity; protein binding; ErbB-3 class receptor binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; cytoskeletal protein binding; tau-protein kinase activity; kinase activity; ATP binding

Biological Process: Schwann cell development; negative regulation of synaptic plasticity; regulation of cell cycle; cerebellar cortex formation; rhythmic process; regulation of synaptic plasticity; motor axon guidance; sensory perception of pain; protein amino acid phosphorylation; receptor clustering; regulation of cell migration; corpus callosum development; neuron differentiation; cerebellar cortex development; receptor catabolic process; cerebellum development; oligodendrocyte differentiation; neurite development; central nervous system neuron development; synaptic transmission, glutamatergic; nervous system development; skeletal muscle development; dendrite morphogenesis; regulation of postsynaptic membrane potential; cell division; synaptic vesicle endocytosis; negative regulation of protein ubiquitination; cerebral cortex development; negative regulation of transcription, DNA-dependent; synaptic transmission, dopaminergic; regulated secretory pathway; exocytosis; negative regulation of protein export from nucleus; positive regulation of protein binding; apoptosis; protein amino acid autophosphorylation; cell-matrix adhesion; neuron migration; nucleocytoplasmic transport; negative regulation of axon extension; negative regulation of cell cycle; intracellular protein transport; synaptogenesis; behavioral response to cocaine; positive regulation of neuron apoptosis; forebrain development; response to wounding; visual learning; cortical actin cytoskeleton organization and biogenesis; layer formation in the cerebral cortex; associative learning; serine phosphorylation of STAT3 protein; negative regulation of proteolysis; cell migration; hippocampus development; peptidyl-threonine phosphorylation; response to cocaine; cell cycle; positive regulation of calcium ion-dependent exocytosis; telencephalon development; neuron apoptosis; peptidyl-serine phosphorylation; axonogenesis; positive regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; phosphorylation; neurite morphogenesis