Rabbit anti-Human, Mouse RT33 Polyclonal Antibody | anti-RT33 antibody

RT33, CT (MRPS33, 28S ribosomal protein S33, mitochondrial) (Biotin)

Cat. Num. AAA6344273

• Gene Names: MRPS33; S33mt; PTD003; CGI-139; MRP-S33
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RT33; Polyclonal Antibody; CT (MRPS33; 28S ribosomal protein S33; mitochondrial) (Biotin); MRPS33; mitochondrial; anti-RT33 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RT33 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RT33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the C-terminal region of human RT33.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RT33 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane).RT33 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(RT33 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT33 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RT33 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RT33 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans.

Product Categories/Family for anti-RT33 antibody

Antibodies; Mitochondrial Proteins

References

Tsuritani, K., et al. Genome Res. 17(7):1005-1014(2007). Zhang, Z., et al. Genomics 81(5):468-480(2003). Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7706349
• NCBI GeneID: 51650
• NCBI Accession #: NP_057155.1
• NCBI GenBank Nucleotide #: NM_016071.3
• UniProt Accession #: Q9Y291
• Molecular Weight: 12,629 Da
• NCBI Official Full Name: 28S ribosomal protein S33, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S33
• NCBI Official Symbol: MRPS33
• NCBI Official Synonym Symbols: S33mt; PTD003; CGI-139; MRP-S33
• NCBI Protein Information: 28S ribosomal protein S33, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S33, mitochondrial
• UniProt Gene Name: MRPS33
• UniProt Synonym Gene Names: MRP-S33; S33mt
• UniProt Entry Name: RT33_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS33: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.

Protein type: Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: mitochondrial inner membrane; mitochondrial small ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: mitochondrial translation; translation; organelle organization and biogenesis