CDK1 sirna

CDK1 siRNA (Mouse)

Cat. Num. AAA8213550

• Gene Names: Cdk1; Cdc2; Cdc2a; p34<CDC2>
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK1; CDK1 siRNA (Mouse); CDC2; CDC2A; CDKN1; Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CDK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 297

• Applicable Applications for CDK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CDK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK1 sirna

siRNA to inhibit CDK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542366
• NCBI GeneID: 12534
• NCBI Accession #: NP_031685.2
• NCBI GenBank Nucleotide #: NM_007659.3
• UniProt Accession #: P11440; P70337; Q3TI12
• Molecular Weight: 34,107 Da
• NCBI Official Full Name: cyclin-dependent kinase 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: Cdk1
• NCBI Official Synonym Symbols: Cdc2; Cdc2a; p34<CDC2>
• NCBI Protein Information: cyclin-dependent kinase 1
• UniProt Protein Name: Cyclin-dependent kinase 1
• UniProt Gene Name: Cdk1
• UniProt Synonym Gene Names: Cdc2; Cdc2a; Cdkn1; CDK1
• UniProt Entry Name: CDK1_MOUSE

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.22; EC 2.7.11.23; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Motility/polarity/chemotaxis; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily

Cellular Component: centrosome; cytoskeleton; mitochondrion; membrane; spindle microtubule; cytoplasm; midbody; nucleus

Molecular Function: RNA polymerase subunit kinase activity; transferase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; histone kinase activity; kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity

Biological Process: mitosis; organ regeneration; positive regulation of protein import into nucleus, translocation; apoptosis; histone phosphorylation; peptidyl-threonine phosphorylation; cell aging; positive regulation of mitotic cell cycle; response to organic nitrogen; cell cycle; protein amino acid phosphorylation; chromosome condensation; peptidyl-serine phosphorylation; response to ethanol; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; protein complex assembly; phosphorylation; positive regulation of DNA replication; negative regulation of apoptosis; positive regulation of cardiac muscle cell proliferation