CD47 sirna

CD47 siRNA (Mouse)

Cat. Num. AAA8208405

• Gene Names: Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CD47; CD47 siRNA (Mouse); Leukocyte surface antigen CD47; Integrin-associated protein; IAP; CD antigen CD47; CD47 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CD47 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 324

• Applicable Applications for CD47 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CD47 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CD47 sirna

siRNA to inhibit CD47 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754382
• NCBI GeneID: 16423
• NCBI Accession #: NP_034711.1
• NCBI GenBank Nucleotide #: NM_010581.3
• UniProt Accession #: Q61735; Q921Z2
• Molecular Weight: 35,317 Da
• NCBI Official Full Name: leukocyte surface antigen CD47
• NCBI Official Synonym Full Names: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• NCBI Official Symbol: Cd47
• NCBI Official Synonym Symbols: IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
• NCBI Protein Information: leukocyte surface antigen CD47
• UniProt Protein Name: Leukocyte surface antigen CD47
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: Cd47
• UniProt Synonym Gene Names: IAP
• UniProt Entry Name: CD47_MOUSE

Uniprot Description

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding

Biological Process: cell migration; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; signal transduction; cell adhesion; positive regulation of inflammatory response