CCM2 sirna

CCM2 siRNA (Mouse)

Cat. Num. AAA8207998

• Gene Names: Ccm2; BC029157
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCM2; CCM2 siRNA (Mouse); OSM; Malcavernin; Cerebral cavernous malformations protein 2 homolog; Osmosensing scaffold for MEKK3; CCM2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CCM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 453

• Applicable Applications for CCM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CCM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCM2 sirna

siRNA to inhibit CCM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22122481
• NCBI GeneID: 216527
• NCBI Accession #: NP_666126.1
• NCBI GenBank Nucleotide #: NM_146014.3
• UniProt Accession #: Q8K2Y9; Q5SUA3
• Molecular Weight: 48,248 Da
• NCBI Official Full Name: cerebral cavernous malformations protein 2 homolog isoform 1
• NCBI Official Synonym Full Names: cerebral cavernous malformation 2
• NCBI Official Symbol: Ccm2
• NCBI Official Synonym Symbols: BC029157
• NCBI Protein Information: cerebral cavernous malformations protein 2 homolog
• UniProt Protein Name: Cerebral cavernous malformations protein 2 homolog
• Protein Family: Cerebral cavernous malformations protein
• UniProt Gene Name: Ccm2
• UniProt Synonym Gene Names: Osm
• UniProt Entry Name: CCM2_MOUSE

Uniprot Description

CCM2: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions. May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3- dependent p38 activation induced by hyperosmotic shock. Defects in CCM2 are the cause of cerebral cavernous malformations type 2 (CCM2). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and are usually present clinically during the 3rd to 5th decades of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Cellular Component: protein complex; cytoplasm

Molecular Function: protein binding

Biological Process: blood vessel development; pericardium development; intercellular junction assembly and maintenance; venous blood vessel morphogenesis; vasculature development; in utero embryonic development; multicellular organism growth; heart development; vasculogenesis; inner ear development; endothelial cell development