Typical Testing Data/Standard Curve (for reference only)
Mouse Matrix metalloproteinase 9 ELISA Kit | MMP-9 elisa kit
Mouse Matrix metalloproteinase 9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MMP-9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MMP-9 antibody and an MMP-9-HRP conjugate. The assay sample and buffer are incubated together with MMP-9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP-9 concentration since MMP-9 from samples and MMP-9-HRP conjugate compete for the anti-MMP-9 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP-9 from the sample, fewer sites are left to bind MMP-9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP-9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.
Protein type: Secreted, signal peptide; EC 3.4.24.35; Secreted; Protease; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity
Biological Process: positive regulation of keratinocyte migration; axon guidance; ossification; extracellular matrix organization and biogenesis; macrophage differentiation; positive regulation of apoptosis; proteolysis; collagen catabolic process; extracellular matrix disassembly; ephrin receptor signaling pathway; leukocyte migration; skeletal development; embryo implantation
Disease: Metaphyseal Anadysplasia 2
Research Articles on MMP-9
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Product Notes
The Mouse MMP-9 mmp9 (Catalog #AAA720876) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720876 ELISA Kit recognizes Mouse MMP-9. It is sometimes possible for the material contained within the vial of "Matrix metalloproteinase 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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