CAD sirna

CAD siRNA (Mouse)

Cat. Num. AAA8208077

• Gene Names: Cad; Cpad; AU018859; 2410008J01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CAD; CAD siRNA (Mouse); CAD protein; CAD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CAD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2154

• Applicable Applications for CAD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CAD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CAD sirna

siRNA to inhibit CAD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 575501556
• NCBI GeneID: 69719
• NCBI Accession #: NP_001276451.1
• NCBI GenBank Nucleotide #: NM_001289522.1
• UniProt Accession #: B2RQC6; B7ZN27
• Molecular Weight: 235,851 Da
• NCBI Official Full Name: CAD protein isoform 2
• NCBI Official Synonym Full Names: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• NCBI Official Symbol: Cad
• NCBI Official Synonym Symbols: Cpad; AU018859; 2410008J01Rik
• NCBI Protein Information: CAD protein
• UniProt Protein Name: CAD protein
• Protein Family: Cadherin
• UniProt Gene Name: Cad
• UniProt Entry Name: PYR1_MOUSE

Uniprot Description

CAD: a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine.

Protein type: EC 3.5.2.3; Nucleotide Metabolism - pyrimidine; Ligase; Hydrolase; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.1.3.2; EC 6.3.5.5

Cellular Component: nucleoplasm; protein complex; nuclear matrix; cell projection; cell soma; membrane; cytoplasm; plasma membrane; terminal button; nucleus; cytosol

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides; identical protein binding; carbamoyl-phosphate synthase (ammonia) activity; zinc ion binding; hydrolase activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; metal ion binding; nucleotide binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; protein kinase activity; transferase activity; amino acid binding; enzyme binding; carboxyl- or carbamoyltransferase activity; aspartate carbamoyltransferase activity; catalytic activity; ATP binding; dihydroorotase activity; ligase activity

Biological Process: amino acid metabolic process; 'de novo' pyrimidine base biosynthetic process; nitrogen compound metabolic process; arginine biosynthetic process; metabolic process; UTP biosynthetic process; protein amino acid autophosphorylation; glutamine catabolic process; peptidyl-threonine phosphorylation; drug metabolic process; urea cycle; pyrimidine nucleotide biosynthetic process