BNIP3L sirna

BNIP3L siRNA (Mouse)

Cat. Num. AAA8229264

• Gene Names: Bnip3l; Nix; Nip3L; C86132; D14Ertd719e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BNIP3L; BNIP3L siRNA (Mouse); NIX; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; NIP3-like protein X; NIP3L; BNIP3L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BNIP3L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 218

• Applicable Applications for BNIP3L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BNIP3L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BNIP3L sirna

siRNA to inhibit BNIP3L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6753200
• NCBI GeneID: 12177
• NCBI Accession #: NP_033891.1
• NCBI GenBank Nucleotide #: NM_009761.3
• UniProt Accession #: Q9Z2F7; Q545J6
• Molecular Weight: 23,766 Da
• NCBI Official Full Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• NCBI Official Synonym Full Names: BCL2/adenovirus E1B interacting protein 3-like
• NCBI Official Symbol: Bnip3l
• NCBI Official Synonym Symbols: Nix; Nip3L; C86132; D14Ertd719e
• NCBI Protein Information: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• UniProt Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Protein Family: BCL2/adenovirus E1B 19 kDa protein-interacting protein
• UniProt Gene Name: Bnip3l
• UniProt Synonym Gene Names: Nix; NIP3L
• UniProt Entry Name: BNI3L_MOUSE

Uniprot Description

BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.

Protein type: Membrane protein, integral; Mitochondrial; Endoplasmic reticulum; Apoptosis; Nuclear envelope

Cellular Component: mitochondrial envelope; mitochondrial outer membrane; membrane; mitochondrion; endoplasmic reticulum; integral to membrane; nuclear envelope; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding

Biological Process: apoptosis; positive regulation of apoptosis; negative regulation of programmed cell death; defense response to virus; negative regulation of apoptosis