Rabbit FIP1L1 Polyclonal Antibody | anti-FIP1L1 antibody

FIP1L1 Polyclonal Antibody

Cat. Num. AAA9433966

• Gene Names: FIP1L1; Rhe; FIP1; hFip1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: FIP1L1; Polyclonal Antibody; FIP1L1 Polyclonal Antibody; FIP1; Rhe; hFip1; pre-mRNA 3'-end-processing factor FIP1; anti-FIP1L1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-FIP1L1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:100
• Immunogen: Recombinant fusion protein of human FIP1L1 (NP_001128409.1).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FIP1L1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using FIP1L1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells using FIP1L1 antibody.)

Related Product Information for anti-FIP1L1 antibody

This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly (A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-FIP1L1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 201023339
• NCBI GeneID: 81608
• NCBI Accession #: NP_001128409.1
• NCBI GenBank Nucleotide #: NM_001134937.1
• UniProt Accession #: Q6UN15; Q0VGE0; Q499Y4; Q49AU3; Q7Z608; Q8WVN3; Q96F80; Q9H077; B4DIR3; G3XAD6
• Molecular Weight: 65,728 Da
• NCBI Official Full Name: pre-mRNA 3'-end-processing factor FIP1 isoform 2
• NCBI Official Synonym Full Names: factor interacting with PAPOLA and CPSF1
• NCBI Official Symbol: FIP1L1
• NCBI Official Synonym Symbols: Rhe; FIP1; hFip1
• NCBI Protein Information: pre-mRNA 3'-end-processing factor FIP1
• UniProt Protein Name: Pre-mRNA 3'-end-processing factor FIP1
• Protein Family: Pre-mRNA 3'-end-processing factor
• UniProt Gene Name: FIP1L1
• UniProt Synonym Gene Names: FIP1; RHE; hFip1
• UniProt Entry Name: FIP1_HUMAN

NCBI Description

This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

FIP1L1: a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre- mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex. A fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA) is the cause of some cases of hypereosinophilic syndrome (HES), a disorder characterized by overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage. Four alternatively spliced isoforms have been described.

Protein type: RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; mRNA cleavage and polyadenylation specificity factor complex

Biological Process: mRNA processing

Disease: Hypereosinophilic Syndrome, Idiopathic