Western Blot (WB)
(Western blot analysis of PRDM10 using K562 whole cell lysates)
Rabbit PRDM10 Polyclonal Antibody | anti-PRDM10 antibody
PRDM10 Antibody
Gene Names
PRDM10; PFM7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PRDM10; Polyclonal Antibody; PRDM10 Antibody; KIAA1231; MGC131802; PFM7; PR domain containing 10; PR domain containing protein 10; PR domain family member 7; PR domain zinc finger protein 10; PR domain-containing protein 10; PRD10_HUMAN; PRDM zinc finger transcription factor; prdm10; TRIS; Tristanin; anti-PRDM10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PRDM10 antibody detects endogenous levels of total PRDM10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1160
Applicable Applications for anti-PRDM10 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human PRDM10
Subcellular Location
Nucleus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PRDM10 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of PRDM10 using K562 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610089 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610089 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-PRDM10 antibody
Function: May be involved in transcriptional regulation.
Similarity: The SET domain is degenerated, suggesting that it has lost methyltransferase activity. Belongs to the class V-like SAM-binding methyltransferase superfamily.
Similarity: The SET domain is degenerated, suggesting that it has lost methyltransferase activity. Belongs to the class V-like SAM-binding methyltransferase superfamily.
References
Zamanian Azodi M, Rezaei Tavirani M, Rezaei Tavirani M, Vafaee R, Rostami-Nejad M; Journal: Asian Pac J Cancer Prev. Nasopharyngeal Carcinoma Protein Interaction Mapping Analysis via Proteomic Approaches.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 130 kDa
Predicted: 131 kDa
Predicted: 131 kDa
NCBI Official Full Name
PR domain zinc finger protein 10 isoform 1
NCBI Official Synonym Full Names
PR/SET domain 10
NCBI Official Symbol
PRDM10
NCBI Official Synonym Symbols
PFM7
NCBI Protein Information
PR domain zinc finger protein 10
UniProt Protein Name
PR domain zinc finger protein 10
Protein Family
UniProt Gene Name
PRDM10
UniProt Synonym Gene Names
KIAA1231; PFM7; TRIS
NCBI Description
The protein encoded by this gene is a transcription factor that contains C2H2-type zinc-fingers. It also contains a positive regulatory domain, which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
May be involved in transcriptional regulation.
Research Articles on PRDM10
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Product Notes
The PRDM10 prdm10 (Catalog #AAA9610089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRDM10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the PRDM10 prdm10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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