BMP7 sirna

BMP7 siRNA (Mouse)

Cat. Num. AAA8225798

• Gene Names: Bmp7; OP1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMP7; BMP7 siRNA (Mouse); BMP-7; OP1; Bone morphogenetic protein 7; Osteogenic protein 1; OP-1; BMP7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BMP7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 430

• Applicable Applications for BMP7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BMP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMP7 sirna

siRNA to inhibit BMP7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31982487
• NCBI GeneID: 12162
• NCBI Accession #: NP_031583.2
• NCBI GenBank Nucleotide #: NM_007557.3
• UniProt Accession #: P23359; Q91XF7
• Molecular Weight: 49,199 Da
• NCBI Official Full Name: bone morphogenetic protein 7 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 7
• NCBI Official Symbol: Bmp7
• NCBI Official Synonym Symbols: OP1
• NCBI Protein Information: bone morphogenetic protein 7
• UniProt Protein Name: Bone morphogenetic protein 7
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp7
• UniProt Synonym Gene Names: Bmp-7; Op1; BMP-7; OP-1
• UniProt Entry Name: BMP7_MOUSE

NCBI Description

This gene encodes a member of a family of secreted factors that participate in developmental signal transduction to stimulate SMAD transcriptional activators. Mutation of this gene results in skeletal, kidney, and other developmental defects. The propeptide is further cleaved into an active chain. [provided by RefSeq, Mar 2013]

Uniprot Description

BMP7: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1. Expressed in the kidney and bladder. Lower levels seen in the brain. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; extracellular space; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: axon guidance; negative regulation of MAP kinase activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; embryonic pattern specification; negative regulation of cell cycle; negative regulation of neurogenesis; BMP signaling pathway; negative regulation of cell proliferation; epithelial cell differentiation; ureteric bud development; salivary gland morphogenesis; anatomical structure formation; kidney development; cell differentiation; embryonic limb morphogenesis; ossification; positive regulation of heterotypic cell-cell adhesion; regulation of phosphorylation; positive regulation of bone mineralization; pattern specification process; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; organ morphogenesis; negative regulation of neuron differentiation; mesenchymal cell differentiation; camera-type eye morphogenesis; branching morphogenesis of a tube; eye development; mesoderm formation; regulation of MAPKKK cascade; cartilage development; negative regulation of mitosis; negative regulation of Notch signaling pathway; negative regulation of phosphorylation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; negative regulation of transcription, DNA-dependent; positive regulation of cell differentiation; positive regulation of neuron differentiation; tube morphogenesis; cell development; neurite morphogenesis; growth