ATG4A sirna

ATG4A siRNA (Mouse)

Cat. Num. AAA8226665

• Gene Names: Atg4a; Apg4a; Autl2; Atg4al; AI627006; AV169859
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATG4A; ATG4A siRNA (Mouse); APG4A; AUTL2; Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagin-2; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; ATG4A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATG4A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 396

• Applicable Applications for ATG4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATG4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATG4A sirna

siRNA to inhibit ATG4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 74136555
• NCBI GeneID: 666468
• NCBI Accession #: NP_777364.3
• NCBI GenBank Nucleotide #: NM_174875.3
• UniProt Accession #: Q8C9S8; Q811B8
• Molecular Weight: 45,095 Da
• NCBI Official Full Name: cysteine protease ATG4A
• NCBI Official Synonym Full Names: autophagy related 4A, cysteine peptidase
• NCBI Official Symbol: Atg4a
• NCBI Official Synonym Symbols: Apg4a; Autl2; Atg4al; AI627006; AV169859
• NCBI Protein Information: cysteine protease ATG4A
• UniProt Protein Name: Cysteine protease ATG4A
• Protein Family: Cysteine protease
• UniProt Gene Name: Atg4a
• UniProt Synonym Gene Names: Apg4a; Autl2
• UniProt Entry Name: ATG4A_MOUSE

Uniprot Description

ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Autophagy; EC 3.4.22.-; Microtubule-binding

Cellular Component: cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; cellular response to nitrogen starvation; autophagic vacuole formation