Western Blot (WB)
(Western Blot (WB) analysis of ATG4A pAb at 1:2000 dilutionLane1:CT26 whole cell lysate (20ug) Lane2:PC12 whole cell lysate (20ug) Lane3:HCT116 whole cell lysate (20ug) Lane4:PC3 whole cell lysate (20ug))
Rabbit ATG4A Polyclonal Antibody | anti-ATG4A antibody
ATG4A Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ATG4A; Polyclonal Antibody; ATG4A Polyclonal Antibody; Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A_HUMAN; Atg4al; AUT like 2 cysteine endopeptidase; AUT-like 2 cysteine endopeptidase; Autl2; Autophagin 2; Autophagin-2; Autophagy related 4A cysteine peptidase; Autophagy related cysteine endopeptidase 2; Autophagy related protein 4 homolog A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; AV169859; Cysteine protease ATG4A; hAPG4A; anti-ATG4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATG4A polyclonal antibody detects endogenous levels of ATG4A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-ATG4A antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:2000
Immunogen
Recombination protein, corresponding to amino acids 38-186 of Human ATG4A.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of ATG4A pAb at 1:2000 dilutionLane1:CT26 whole cell lysate (20ug) Lane2:PC12 whole cell lysate (20ug) Lane3:HCT116 whole cell lysate (20ug) Lane4:PC3 whole cell lysate (20ug))
Western Blot (WB)
(Western Blot (WB) analysis of ATG4A pAb at 1:2000 dilutionLane1:CT26 whole cell lysate (20ug) Lane2:PC12 whole cell lysate (20ug) Lane3:HCT116 whole cell lysate (20ug) Lane4:PC3 whole cell lysate (20ug))
Related Product Information for anti-ATG4A antibody
Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4a (ATG4 autophagy related 4 homolog A), also known as APG4A or AUTL2, is a 398 amino acid protein that localizes to the cytoplasm and belongs to the peptidase C54 family. Expressed in a variety of tissues, including brain, skeletal muscle and fetal liver, Atg4a functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4a exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The ATG4A (Catalog #AAA3011264) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG4A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG4A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:2000. Researchers should empirically determine the suitability of the ATG4A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
