ASXL1 sirna

ASXL1 siRNA (Mouse)

Cat. Num. AAA8234544

• Gene Names: Asxl1; mKIAA0978
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASXL1; ASXL1 siRNA (Mouse); KIAA0978; Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ASXL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1514

• Applicable Applications for ASXL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ASXL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASXL1 sirna

siRNA to inhibit ASXL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 90568303
• NCBI GeneID: 228790
• NCBI Accession #: NP_001035028.1
• NCBI GenBank Nucleotide #: NM_001039939.1
• UniProt Accession #: P59598
• Molecular Weight: 162,674 Da
• NCBI Official Full Name: putative Polycomb group protein ASXL1
• NCBI Official Synonym Full Names: additional sex combs like 1
• NCBI Official Symbol: Asxl1
• NCBI Official Synonym Symbols: mKIAA0978
• NCBI Protein Information: putative Polycomb group protein ASXL1
• UniProt Protein Name: Putative Polycomb group protein ASXL1
• Protein Family: Putative Polycomb group protein
• UniProt Gene Name: Asxl1
• UniProt Synonym Gene Names: Kiaa0978
• UniProt Entry Name: ASXL1_MOUSE

Uniprot Description

ASXL1: Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG). Acts as coactivator of RARA and RXRA through association with NCOA1. Acts as corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner; the function seems to involve differential recruitment of methylated histone H3 to respective promoters. Acts as corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Defects in ASXL1 are the cause of Bohring-Opitz syndrome (BOPS). A syndrome characterized by severe intrauterine growth retardation, poor feeding, profound mental retardation, trigonocephaly, prominent metopic suture, exophthalmos, nevus flammeus of the face, upslanting palpebral fissures, hirsutism, and flexion of the elbows and wrists with deviation of the wrists and metacarpophalangeal joints. Defects in ASXL1 are a cause of myelodysplastic syndrome (MDS). A heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS); chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. MDS is considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Belongs to the Asx family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Nuclear receptor co-regulator

Cellular Component: nuclear chromatin; nucleus

Molecular Function: peroxisome proliferator activated receptor binding; protein binding; DNA binding; retinoic acid receptor binding; metal ion binding; transcription coactivator activity; transcription corepressor activity

Biological Process: heart morphogenesis; negative regulation of retinoic acid receptor signaling pathway; response to retinoic acid; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of retinoic acid receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation