Rabbit anti-Human, Mouse ATG2A Polyclonal Antibody | anti-ATG2A antibody
Rabbit Anti-Human ATG2A Polyclonal
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5002 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.)
Western Blot (WB)
(Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody . Lane 1: MW Ladder. Lane 2: Mouse Brain (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.)
Western Blot (WB)
(Western blot analysis of Human HeLa cell lysates showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody . Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.)
NCBI and Uniprot Product Information
Uniprot Description
ATG2A: Belongs to the ATG2 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: extrinsic to membrane
Molecular Function: protein binding
Biological Process: autophagic vacuole formation; mitochondrion degradation
Research Articles on ATG2A
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Product Notes
The ATG2A atg2a (Catalog #AAA807445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human ATG2A Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000. Researchers should empirically determine the suitability of the ATG2A atg2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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