ASH1L sirna

ASH1L siRNA (Mouse)

Cat. Num. AAA8224962

• Gene Names: Ash1l; Ash1; Kmt2h; 8030453L17Rik; E430018P19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ASH1L; ASH1L siRNA (Mouse); Histone-lysine N-methyltransferase ASH1L; ASH1-like protein; Absent small and homeotic disks protein 1 homolog; ASH1L sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ASH1L siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2958

• Applicable Applications for ASH1L sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ASH1L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ASH1L sirna

siRNA to inhibit ASH1L expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 73622271
• NCBI GeneID: 192195
• NCBI Accession #: NP_619620.3
• NCBI GenBank Nucleotide #: NM_138679.5
• UniProt Accession #: Q99MY8; Q3U598; Q80VY5; Q8BM69; Q8BTX0; Q8BZY6; E9QNM2
• Molecular Weight: 331,333 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase ASH1L
• NCBI Official Synonym Full Names: ash1 (absent, small, or homeotic)-like (Drosophila)
• NCBI Official Symbol: Ash1l
• NCBI Official Synonym Symbols: Ash1; Kmt2h; 8030453L17Rik; E430018P19Rik
• NCBI Protein Information: histone-lysine N-methyltransferase ASH1L
• UniProt Protein Name: Histone-lysine N-methyltransferase ASH1L
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Ash1l
• UniProt Entry Name: ASH1L_MOUSE

Uniprot Description

ASH1L: a protein methyltransferase that specifically methylates K36 of histone H3 (H3K36me). A member of the trithorax group (trxG) of transcriptional activators that is required for proper expression of Hox genes and is preferentially expressed in haematopoietic stem cells. Widely expressed, with high levels in brain, heart and kidney. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Cell adhesion; Methyltransferase

Cellular Component: nucleoplasm; Golgi apparatus; tight junction; cytoplasm; chromosome; nucleus; cell junction

Molecular Function: transferase activity; methyltransferase activity; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding; chromatin binding; histone-lysine N-methyltransferase activity

Biological Process: methylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of MAPKKK cascade; interleukin-6 production; negative regulation of inflammatory response; histone H3-K4 methylation; negative regulation of acute inflammatory response; positive regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of I-kappaB kinase/NF-kappaB cascade; peptidyl-lysine methylation