ACVR1B sirna

ACVR1B siRNA (Mouse)

Cat. Num. AAA8241054

• Gene Names: Acvr1b; Alk4; SKR2; ActRIB; ActR-IB; Acvrlk4; 6820432J04
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACVR1B; ACVR1B siRNA (Mouse); ALK4; Activin receptor type-1B; Activin receptor type IB; ACTR-IB; Activin receptor-like kinase 4; ALK-4; Serine/threonine-protein kinase receptor R2; SKR2; ACVR1B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACVR1B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 505

• Applicable Applications for ACVR1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACVR1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACVR1B sirna

siRNA to inhibit ACVR1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6680630
• NCBI GeneID: 11479
• NCBI Accession #: NP_031421.1
• NCBI GenBank Nucleotide #: NM_007395.3
• UniProt Accession #: Q61271
• Molecular Weight: 56,700 Da
• NCBI Official Full Name: activin receptor type-1B
• NCBI Official Synonym Full Names: activin A receptor, type 1B
• NCBI Official Symbol: Acvr1b
• NCBI Official Synonym Symbols: Alk4; SKR2; ActRIB; ActR-IB; Acvrlk4; 6820432J04
• NCBI Protein Information: activin receptor type-1B
• UniProt Protein Name: Activin receptor type-1B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr1b
• UniProt Synonym Gene Names: Alk4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_MOUSE

Uniprot Description

ALK4: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine- threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C- terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. ACVRIB is abundantly expressed in systemic sclerosis patient fibroblasts and production of collagen is also induced by activin-A/INHBA. This suggests that the activin/ACRV1B signaling mechanism is involved in systemic sclerosis. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Membrane protein, integral; Kinase, protein; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; TKL group; STKR family; Type1 subfamily

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane; integral to membrane; activin receptor complex; receptor complex

Molecular Function: activin receptor activity; metal ion binding; nucleotide binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; growth factor binding; transferase activity, transferring phosphorus-containing groups; activin binding; kinase activity; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: in utero embryonic development; positive regulation of erythrocyte differentiation; protein amino acid autophosphorylation; activin receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of signal transduction; signal transduction; protein amino acid phosphorylation; positive regulation of activin receptor signaling pathway; embryonic development; cell surface receptor linked signal transduction; hair follicle development; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; phosphorylation; G1/S transition of mitotic cell cycle