ALK4/ACVR1B Active Protein | ACVR1B active protein

ALK4/ACVR1B Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8123159

• Gene Names: Acvr1b; Alk4; SKR2; ActRIB; ActR-IB; Acvrlk4; 6820432J04
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ALK4/ACVR1B; ALK4/ACVR1B Protein; Mouse; Recombinant (hFc Tag); Mouse ALK-4/ACVR1B Protein (Fc Tag); 6820432J04 Protein; ActR-IB Protein; ActRIB Protein; Acvrlk4 Protein; Alk4 Protein; SKR2 Protein; activin A receptor; type IB; ACVR1B active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu126

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Acvr1b (NP_031421.1)(Met1-Glu126) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to bind with rat TDGF1-His in a functional ELISA.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ACVR1B active protein

Background: ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

References

Chen Y, et al. (2005) Developmental analysis of activin-like kinase receptor-4 (ALK4) expression in Xenopus laevis. 232(2): 393-8.J. Massagu. (1998) TGF- SIGNAL TRANSDUCTION. Annual Review of Biochemistry. 67: 753-91.

NCBI and Uniprot Product Information

• NCBI GI #: 6680630
• NCBI GeneID: 11479
• NCBI Accession #: NP_031421.1
• NCBI GenBank Nucleotide #: NM_007395.3
• UniProt Accession #: Q61271
• Molecular Weight: 56,700 Da
• NCBI Official Full Name: activin receptor type-1B
• NCBI Official Synonym Full Names: activin A receptor, type 1B
• NCBI Official Symbol: Acvr1b
• NCBI Official Synonym Symbols: Alk4; SKR2; ActRIB; ActR-IB; Acvrlk4; 6820432J04
• NCBI Protein Information: activin receptor type-1B; ALK-4; activin receptor type IB; activin receptor-like kinase 4; serine/threonine-protein kinase receptor R2
• UniProt Protein Name: Activin receptor type-1B
• Protein Family: Activin receptor
• UniProt Gene Name: Acvr1b
• UniProt Synonym Gene Names: Alk4; ACTR-IB; ALK-4; SKR2
• UniProt Entry Name: ACV1B_MOUSE

Uniprot Description

Function: Transmembrane serine/threonine kinase activin type-1 receptor forming an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating a many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, type-2 receptors (ACVR2A and/or ACVR2B) act as a primary activin receptors whereas the type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor such as ACVR1B. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor. ACVR1B also phosphorylates TDP2. Ref.2 Ref.3

Catalytic activity: ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Enzyme regulation: Activin receptor type-2 (ACVR2A or ACVR2B) activates the type-1 receptor through phosphorylation of its regulatory GS domain

By similarity.

Subunit structure: Forms an activin receptor complex with activin receptor type-2 (ACVR2A or ACVR2B). Interacts with TDP2

By similarity. Interacts with AIP1, FKBP1A, IGSF1, TDGF1, SMAD2, SMAD3 and SMAD7

By similarity. Ref.3

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity.

Domain: The GS domain is a 30-amino-acid sequence adjacent to the N-terminal boundary of the kinase domain and highly conserved in all other known type-1 receptors but not in type-2 receptors. The GS domain is the site of activation through phosphorylation by the II receptors

By similarity.

Post-translational modification: Autophosphorylated. Phosphorylated by activin receptor type-2 (ACVR2A or ACVR2B) in response to activin-binding at serine and threonine residues in the GS domain. Phosphorylation of ACVR1B by activin receptor type-2 regulates association with SMAD7

By similarity.Ubiquitinated. Level of ubiquitination is regulated by the SMAD7-SMURF1 complex

By similarity.Ubiquitinated

By similarity.

Disruption phenotype: Leads to hair loss. Ref.4

Sequence similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.Contains 1 GS domain.Contains 1 protein kinase domain.