ACTB sirna

ACTB siRNA (Mouse)

Cat. Num. AAA8224213

• Gene Names: Actb; Actx; beta-actin; E430023M04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACTB; ACTB siRNA (Mouse); Actin cytoplasmic 1; Beta-actin; ACTB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACTB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 375

• Applicable Applications for ACTB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACTB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACTB sirna

siRNA to inhibit ACTB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6671509
• NCBI GeneID: 11461
• NCBI Accession #: NP_031419.1
• NCBI GenBank Nucleotide #: NM_007393.4
• UniProt Accession #: P60710; P02570; P70514; P99021; Q11211; Q3TI89; Q3TVP6; Q64316; Q6ZWM3
• Molecular Weight: 41,737 Da
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: Actb
• NCBI Official Synonym Symbols: Actx; beta-actin; E430023M04Rik
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Actin
• UniProt Gene Name: Actb
• UniProt Entry Name: ACTB_MOUSE

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; cytoskeleton; membrane; axon; cytoplasm; postsynaptic density; nuclear chromatin; ribonucleoprotein complex; cytosol; myelin sheath

Molecular Function: protein binding; nucleosomal DNA binding; Tat protein binding; kinesin binding; nucleotide binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axonogenesis; ATP-dependent chromatin remodeling