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Western Blot (WB) (All lanes: Actin, cytoplasmic 1 antibody at 2ug/ml)

Rabbit anti-Mouse Actin 1 Polyclonal Antibody | anti-Actb antibody

Actin, cytoplasmic 1 Polyclonal Antibody

Gene Names
Actb; Actx; beta-actin; E430023M04Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Actin 1; Polyclonal Antibody; Actin; cytoplasmic 1 Polyclonal Antibody; Actb; Beta-actin; anti-Actb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total Actin, cytoplasmic 1 polyclonal antibody.
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
375
Applicable Applications for anti-Actb antibody
Western Blot (WB)
Application Notes
Western Blot: 1:500 - 1:1000
Immunogen
Recombinant mouse Actin, cytoplasmic 1 protein
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(All lanes: Actin, cytoplasmic 1 antibody at 2ug/ml)

Western Blot (WB) (All lanes: Actin, cytoplasmic 1 antibody at 2ug/ml)
Related Product Information for anti-Actb antibody
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Product Categories/Family for anti-Actb antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,737 Da
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
Actb
NCBI Official Synonym Symbols
Actx; beta-actin; E430023M04Rik
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
Protein Family
UniProt Gene Name
Actb
UniProt Entry Name
ACTB_MOUSE

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: axon; cortical cytoskeleton; cytosol; extracellular space; focal adhesion; membrane; myelin sheath; NuA4 histone acetyltransferase complex; nuclear chromatin; podosome; postsynaptic density; protein complex; ribonucleoprotein complex; stress fiber

Molecular Function: identical protein binding; kinesin binding; nitric-oxide synthase binding; nucleosomal DNA binding; protein binding; protein kinase binding; Tat protein binding

Biological Process: ATP-dependent chromatin remodeling; axonogenesis

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Product Notes

The Actb actb (Catalog #AAA9415354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Actin, cytoplasmic 1 Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Actin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:500 - 1:1000. Researchers should empirically determine the suitability of the Actb actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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