AANAT sirna

AANAT siRNA (Mouse)

Cat. Num. AAA8222889

• Gene Names: Aanat; Nat4; Snat; Nat-2; AA-NAT
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AANAT; AANAT siRNA (Mouse); SNAT; Serotonin N-acetyltransferase; Serotonin acetylase; Aralkylamine N-acetyltransferase; AA-NAT; AANAT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AANAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 205

• Applicable Applications for AANAT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AANAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AANAT sirna

siRNA to inhibit AANAT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6752938
• NCBI GeneID: 11298
• NCBI Accession #: NP_033721.1
• NCBI GenBank Nucleotide #: NM_009591.3
• UniProt Accession #: O88816
• Molecular Weight: 23,069 Da
• NCBI Official Full Name: serotonin N-acetyltransferase
• NCBI Official Synonym Full Names: arylalkylamine N-acetyltransferase
• NCBI Official Symbol: Aanat
• NCBI Official Synonym Symbols: Nat4; Snat; Nat-2; AA-NAT
• NCBI Protein Information: serotonin N-acetyltransferase
• UniProt Protein Name: Serotonin N-acetyltransferase
• Protein Family: Serotonin N-acetyltransferase
• UniProt Gene Name: Aanat
• UniProt Synonym Gene Names: Snat; Serotonin acetylase; AA-NAT
• UniProt Entry Name: SNAT_MOUSE

NCBI Description

The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]

Uniprot Description

AA-NAT: Catalyzes the N-acetylation of serotonin into N- acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines. Belongs to the acetyltransferase family. AANAT subfamily. Monomer. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect.

Protein type: Amino Acid Metabolism - tryptophan; Acetyltransferase; EC 2.3.1.87

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: transferase activity; N-acetyltransferase activity; transferase activity, transferring acyl groups; arylamine N-acetyltransferase activity; aralkylamine N-acetyltransferase activity

Biological Process: N-terminal protein amino acid acetylation; circadian rhythm; melatonin biosynthetic process; response to copper ion; response to zinc ion; rhythmic process; response to organic cyclic substance; response to insulin stimulus