Typical Testing Data/Standard Curve
Human Serotonin N acetyltransferase (AANAT) ELISA Kit | AANAT elisa kit
Human Serotonin N acetyltransferase (AANAT) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||AANAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AANAT antibody and an AANAT-HRP conjugate. The assay sample and buffer are incubated together with AANAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AANAT concentration since AANAT from samples and AANAT-HRP conjugate compete for the anti-AANAT antibody binding site. Since the number of sites is limited, as more sites are occupied by AANAT from the sample, fewer sites are left to bind AANAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AANAT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
AA-NAT var1: Catalyzes the N-acetylation of serotonin into N- acetylserotonin. Controls the night/day rhythm of melatonin production in the pineal gland. Has a relative affinity for hydroxylated versus non-hydroxylated arylalkylamines. Belongs to the acetyltransferase family. AANAT subfamily. Monomer. Interacts with YWHAZ; the interaction requires phosphorylation on Thr-31, and modulates the enzymatic activity of AANAT through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently, a second molecule of AANAT can bind, via the phosphorylated Ser-205 site, the other YWHAZ monomer with similar effect.
Protein type: Acetyltransferase; EC 2.3.1.87; Amino Acid Metabolism - tryptophan
Chromosomal Location of Human Ortholog: 17q25.1
Cellular Component: perinuclear region of cytoplasm; cytosol
Molecular Function: arylamine N-acetyltransferase activity; aralkylamine N-acetyltransferase activity
Biological Process: circadian rhythm; N-terminal protein amino acid acetylation; response to light stimulus; melatonin biosynthetic process; response to copper ion; response to zinc ion; indolalkylamine biosynthetic process; response to cytokine stimulus; response to calcium ion; response to corticosterone stimulus; response to insulin stimulus
Disease: Delayed Sleep Phase Syndrome, Susceptibility To
Research Articles on AANAT
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Product Notes
The Human AANAT aanat (Catalog #AAA7208795) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7208795 ELISA Kit recognizes Human AANAT. It is sometimes possible for the material contained within the vial of "Serotonin N acetyltransferase (AANAT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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