ZNF644 sirna

ZNF644 siRNA (Human)

Cat. Num. AAA8204830

• Gene Names: ZNF644; NatF; MYP21; ZEP-2; BM-005
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZNF644; ZNF644 siRNA (Human); KIAA1221; ZEP2; Zinc finger protein 644; Zinc finger motif enhancer-binding protein 2; Zep-2; ZNF644 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZNF644 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 105

• Applicable Applications for ZNF644 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZNF644 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZNF644 sirna

siRNA to inhibit ZNF644 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41152095
• NCBI GeneID: 84146
• NCBI Accession #: NP_057704.2
• NCBI GenBank Nucleotide #: NM_016620.3
• UniProt Accession #: Q9H582; Q2TAM0; Q5TCC0; Q6BEP7; Q6P446; Q6PI06; Q7LG67; Q9ULJ9; A2RU71
• Molecular Weight: 11,724 Da
• NCBI Official Full Name: zinc finger protein 644 isoform 2
• NCBI Official Synonym Full Names: zinc finger protein 644
• NCBI Official Symbol: ZNF644
• NCBI Official Synonym Symbols: NatF; MYP21; ZEP-2; BM-005
• NCBI Protein Information: zinc finger protein 644
• UniProt Protein Name: Zinc finger protein 644
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF644
• UniProt Synonym Gene Names: KIAA1221; ZEP2; Zep-2
• UniProt Entry Name: ZN644_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that may play a role in eye development. Defects in this gene have been associated with high myopia. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

ZNF644: May be involved in transcriptional regulation. Defects in ZNF644 are the cause of myopia type 21 (MYP21). A refractive error of the eye, in which parallel rays from a distant object come to focus in front of the retina, vision being better for near objects than for far. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 1p22.2

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: Myopia 21, Autosomal Dominant