Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin K-dependent protein Z (PROZ) Standard Curve.)
Mouse Vitamin K-Dependent Protein Z (PROZ) ELISA Kit | PROZ elisa kit
Mouse Vitamin K-Dependent Protein Z (PROZ) ELISA Kit
Gene Names
Proz; 1300015B06Rik
Reactivity
Mouse
Synonyms
Vitamin K-Dependent Protein Z (PROZ); Mouse Vitamin K-Dependent Protein Z (PROZ) ELISA Kit; PROZ; PZ; PROZ elisa kit
Reactivity
Mouse
Specificity
Mouse Vitamin K-dependent protein Z (PROZ) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROZ. No significant cross-reactivity or interference between Mouse PROZ and analogues was observed.
Sequence Length
399
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin K-dependent protein Z (PROZ) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Vitamin K-dependent protein Z (PROZ) Standard Curve.)
Related Product Information for PROZ elisa kit
Description: This Mouse Vitamin K-dependent protein Z (PROZ) ELISA Kit employs a two-site sandwich ELISA to quantitate PROZ in samples. An antibody specific for PROZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein Z is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy. It is a glycoprotein. Structural analysis of protein Z will allow better understanding of its function. From a Ramachandran plot, the secondary structure of Protein Z was determined. The Ramachandran plot utilizes mathematical equations to determine the possible angles of the amino acids within the primary sequence of Protein Z. The possible angles results in a plot of possible secondary structures. The Ramachandran plot for protein Z indicates it will form alpha helices. The final structure, all aphla domain, was determined by x-ray diffraction. It consists of chain A and B, which are both helix-loop-helix motifs.
Background: Protein Z is a member of the coagulation cascade, the group of blood proteins that leads to the formation of blood clots. It is vitamin K-dependent, and its functionality is therefore impaired in warfarin therapy. It is a glycoprotein. Structural analysis of protein Z will allow better understanding of its function. From a Ramachandran plot, the secondary structure of Protein Z was determined. The Ramachandran plot utilizes mathematical equations to determine the possible angles of the amino acids within the primary sequence of Protein Z. The possible angles results in a plot of possible secondary structures. The Ramachandran plot for protein Z indicates it will form alpha helices. The final structure, all aphla domain, was determined by x-ray diffraction. It consists of chain A and B, which are both helix-loop-helix motifs.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vitamin K-dependent protein Z isoform 1
NCBI Official Synonym Full Names
protein Z, vitamin K-dependent plasma glycoprotein
NCBI Official Symbol
Proz
NCBI Official Synonym Symbols
1300015B06Rik
NCBI Protein Information
vitamin K-dependent protein Z
UniProt Protein Name
Vitamin K-dependent protein Z
Protein Family
UniProt Gene Name
Proz
Uniprot Description
Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids ().
Research Articles on PROZ
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Product Notes
The Mouse PROZ proz (Catalog #AAA9717254) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717254 ELISA Kit recognizes Mouse PROZ. It is sometimes possible for the material contained within the vial of "Vitamin K-Dependent Protein Z (PROZ), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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