YME1L1 sirna

YME1L1 siRNA (Human)

Cat. Num. AAA8203493

• Gene Names: YME1L1; FTSH; MEG4; PAMP; YME1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YME1L1; YME1L1 siRNA (Human); FTSH1; YME1L; ATP-dependent zinc metalloprotease YME1L1; ATP-dependent metalloprotease FtsH1; Meg-4; Presenilin-associated metalloprotease; PAMP; YME1-like protein 1; YME1L1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: YME1L1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 683

• Applicable Applications for YME1L1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human YME1L1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YME1L1 sirna

siRNA to inhibit YME1L1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 359718987
• NCBI GeneID: 10730
• NCBI Accession #: NP_001240795.1
• NCBI GenBank Nucleotide #: NM_001253866.1
• UniProt Accession #: Q96TA2; Q5T8D9; Q9H1Q0; Q9UMR9; B4DNM1; D3DRV8; D3DRV9
• Molecular Weight: 75,982 Da
• NCBI Official Full Name: ATP-dependent zinc metalloprotease YME1L1 isoform 4
• NCBI Official Synonym Full Names: YME1-like 1 ATPase
• NCBI Official Symbol: YME1L1
• NCBI Official Synonym Symbols: FTSH; MEG4; PAMP; YME1L
• NCBI Protein Information: ATP-dependent zinc metalloprotease YME1L1
• UniProt Protein Name: ATP-dependent zinc metalloprotease YME1L1
• Protein Family: ATP-dependent zinc metalloprotease
• UniProt Gene Name: YME1L1
• UniProt Synonym Gene Names: FTSH1; YME1L; PAMP
• UniProt Entry Name: YMEL1_HUMAN

NCBI Description

The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

YME1L1: Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Protease; EC 3.4.24.-

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: membrane; mitochondrial inner membrane; integral to membrane

Molecular Function: metal ion binding; metalloendopeptidase activity; ATP binding

Biological Process: mitochondrion organization and biogenesis; cell proliferation; misfolded or incompletely synthesized protein catabolic process