Typical Testing Data/Standard Curve (for reference only)
Porcine ATP-dependent zinc metalloprotease YME1L1 (YME1L1) ELISA Kit | YME1L1 elisa kit
Porcine ATP-dependent zinc metalloprotease YME1L1 (YME1L1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: YME1L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YME1L1 antibody and an YME1L1-HRP conjugate. The assay sample and buffer are incubated together with YME1L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YME1L1 concentration since YME1L1 from samples and YME1L1-HRP conjugate compete for the anti-YME1L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YME1L1 from the sample, fewer sites are left to bind YME1L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YME1L1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
YME1L1: Putative ATP-dependent protease which plays a role in mitochondrial protein metabolism. Ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. Requires to control the accumulation of nonassembled respiratory chain subunits (NDUFB6, OX4 and ND1). Seems to act in the processing of OPA1. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.24.-; Protease; Mitochondrial
Chromosomal Location of Human Ortholog: 10p14
Cellular Component: membrane; mitochondrial inner membrane; integral to membrane
Molecular Function: metal ion binding; metalloendopeptidase activity; ATP binding
Biological Process: mitochondrion organization and biogenesis; cell proliferation; misfolded or incompletely synthesized protein catabolic process
Research Articles on YME1L1
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Product Notes
The Porcine YME1L1 yme1l1 (Catalog #AAA7208875) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7208875 ELISA Kit recognizes Porcine YME1L1. It is sometimes possible for the material contained within the vial of "ATP-dependent zinc metalloprotease YME1L1 (YME1L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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