XIAP sirna

XIAP siRNA (Human)

Cat. Num. AAA8218021

• Gene Names: XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: XIAP; XIAP siRNA (Human); API3; BIRC4; IAP3; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked IAP; XIAP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: XIAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 497

• Applicable Applications for XIAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human XIAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for XIAP sirna

siRNA to inhibit XIAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32528299
• NCBI GeneID: 331
• NCBI Accession #: NP_001158.2
• NCBI GenBank Nucleotide #: NM_001167.3
• UniProt Accession #: P98170; Q9NQ14; D3DTF2
• Molecular Weight: 56,685 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase XIAP
• NCBI Official Synonym Full Names: X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase
• NCBI Official Symbol: XIAP
• NCBI Official Synonym Symbols: API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
• NCBI Protein Information: E3 ubiquitin-protein ligase XIAP
• UniProt Protein Name: E3 ubiquitin-protein ligase XIAP
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XIAP
• UniProt Synonym Gene Names: API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3; X-linked IAP
• UniProt Entry Name: XIAP_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

Uniprot Description

XIAP: an apoptotic suppressor protein. Inhibist apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. Inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. Inhibits cell-death proteases, caspase-3, caspase-7, and perhaps caspase-9.

Protein type: Apoptosis; EC 6.3.2.-; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: nucleoplasm; spindle microtubule; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: Wnt receptor signaling pathway; positive regulation of protein ubiquitination; apoptosis; regulation of BMP signaling pathway; regulation of inflammatory response; protein ubiquitination; negative regulation of caspase activity; response to DNA damage stimulus; copper ion homeostasis; regulation of innate immune response; regulation of cell proliferation; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome, X-linked, 1; Lymphoproliferative Syndrome, X-linked, 2