Rabbit PDK2 Polyclonal Antibody | anti-PDK2 antibody

PDK2, CT (PDK2, PDHK2, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2) (Azide free) (HRP)

Cat. Num. AAA6332412

• Gene Names: PDK2; PDHK2; PDKII
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PDK2; Polyclonal Antibody; CT (PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2; mitochondrial; Pyruvate dehydrogenase kinase isoform 2) (Azide free) (HRP); Pyruvate dehydrogenase kinase isoform 2; anti-PDK2 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PDK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378~407 amino acids from the C-term region of human PDK2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PDK2 Antibody (C-term) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(PDK2 Antibody (C392) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PDK2 antibody

PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Product Categories/Family for anti-PDK2 antibody

Antibodies; Protein Kinases

References

Hiromasa, Y., et al., J. Biol. Chem. 278(36):33681-33693 (2003). Baker, J.C., et al., J. Biol. Chem. 275(21):15773-15781 (2000). Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995). Sun, W. et al. Clin Cancer Res. January 15; 15(2): 476?84(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 315630349
• NCBI GeneID: 5164
• NCBI Accession #: NP_001186827.1
• NCBI GenBank Nucleotide #: NM_001199898.1
• UniProt Accession #: Q15119; Q6P515; Q9BS05; A8K3A7; B3KNW0
• Molecular Weight: 46,154 Da
• NCBI Official Full Name: pyruvate dehydrogenase kinase, isozyme 2 isoform 2
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase, isozyme 2
• NCBI Official Symbol: PDK2
• NCBI Official Synonym Symbols: PDHK2; PDKII
• NCBI Protein Information: pyruvate dehydrogenase kinase, isozyme 2; PDH kinase 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
• UniProt Gene Name: PDK2
• UniProt Synonym Gene Names: PDHK2; PDH kinase 2; PDKII
• UniProt Entry Name: PDK2_HUMAN

NCBI Description

This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; Protein kinase, atypical; EC 2.7.11.2; Kinase, protein; ATYPICAL group; PDHK family; PDHK subfamily

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial pyruvate dehydrogenase complex

Molecular Function: protein homodimerization activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: cellular metabolic process; insulin receptor signaling pathway; regulation of pH; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; regulation of gluconeogenesis; cellular response to nutrient; glucose homeostasis; pyruvate metabolic process; protein amino acid phosphorylation