VNN2 sirna

VNN2 siRNA (Human)

Cat. Num. AAA8212636

• Gene Names: VNN2; FOAP-4; GPI-80
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VNN2; VNN2 siRNA (Human); Vascular non-inflammatory molecule 2; Vanin-2; Glycosylphosphatidyl inositol-anchored protein GPI-80; Protein FOAP-4; VNN2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VNN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 299

• Applicable Applications for VNN2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VNN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VNN2 sirna

siRNA to inhibit VNN2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 334278906
• NCBI GeneID: 8875
• NCBI Accession #: NP_001229279.1
• NCBI GenBank Nucleotide #: NM_001242350.2
• UniProt Accession #: O95498; Q2XUN1; Q9UJF3; A0AUZ3; A6NDY1; A8K4E3; A8K7W0; B2DFZ0; B2DFZ1; B2DFZ2; B2DFZ3; F6XL73
• Molecular Weight: 52,844 Da
• NCBI Official Full Name: vascular non-inflammatory molecule 2 isoform 3
• NCBI Official Synonym Full Names: vanin 2
• NCBI Official Symbol: VNN2
• NCBI Official Synonym Symbols: FOAP-4; GPI-80
• NCBI Protein Information: vascular non-inflammatory molecule 2
• UniProt Protein Name: Vascular non-inflammatory molecule 2
• Protein Family: Vascular non-inflammatory molecule
• UniProt Gene Name: VNN2
• UniProt Synonym Gene Names: Vanin-2
• UniProt Entry Name: VNN2_HUMAN

NCBI Description

This gene product is a member of the Vanin family of proteins that share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. The encoded protein is a GPI-anchored cell surface molecule that plays a role in transendothelial migration of neutrophils. This gene lies in close proximity to, and in same transcriptional orientation as two other vanin genes on chromosome 6q23-q24. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

VNN2: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Involved in the thymus homing of bone marrow cells. May regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil. Belongs to the CN hydrolase family. BTD/VNN subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; EC 3.5.1.92

Chromosomal Location of Human Ortholog: 6q23-q24

Cellular Component: plasma membrane

Molecular Function: pantetheine hydrolase activity

Biological Process: pantothenate metabolic process; cell motility