TUBB4A sirna

TUBB4A siRNA (Human)

Cat. Num. AAA828885

• Gene Names: TUBB4A; DYT4; TUBB4; beta-5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TUBB4A; TUBB4A siRNA (Human); TUBB4; TUBB5; Tubulin beta-4A chain; Tubulin 5 beta; Tubulin beta-4 chain; TUBB4A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TUBB4A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 495

• Applicable Applications for TUBB4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TUBB4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TUBB4A sirna

siRNA to inhibit TUBB4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 574584803
• NCBI GeneID: 10382
• NCBI Accession #: NP_001276052.1
• NCBI GenBank Nucleotide #: NM_001289123.1
• UniProt Accession #: P04350; Q969E5; B3KQP4
• Molecular Weight: 49,586 Da
• NCBI Official Full Name: tubulin beta-4A chain isoform 1
• NCBI Official Synonym Full Names: tubulin, beta 4A class IVa
• NCBI Official Symbol: TUBB4A
• NCBI Official Synonym Symbols: DYT4; TUBB4; beta-5
• NCBI Protein Information: tubulin beta-4A chain
• UniProt Protein Name: Tubulin beta-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB4A
• UniProt Synonym Gene Names: TUBB4; TUBB5
• UniProt Entry Name: TBB4A_HUMAN

NCBI Description

This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]

Uniprot Description

TUBB4: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: internode region of axon; microtubule; cell soma; nucleus; cytosol; myelin sheath; cilium

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; transmembrane transport; microtubule-based process

Disease: Dystonia 4, Torsion, Autosomal Dominant