Rabbit anti-Human, Mouse TUBB4A Polyclonal Antibody | anti-TUBB4A antibody

TUBB4A Antibody

Cat. Num. AAA7045158

• Gene Names: TUBB4A; DYT4; TUBB4; beta-5
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: TUBB4A; Polyclonal Antibody; TUBB4A Antibody; Tubulin beta-4A chain; Tubulin beta-4A chain (Tubulin 5 beta) (Tubulin beta-4 chain); TUBB4 TUBB5; anti-TUBB4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-TUBB4A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC:1:20-1:200; IF:1:50-1:200
• Immunogen: Recombinant Human Tubulin beta-4A chain protein (1-444AA)
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Immunogen Species: Homo sapiens (Human)
• Research Area: Tags & Cell Markers
• Target Names: TUBB4A
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes :TUBB4A antibody at 2 ug/mlLane 1 : 293T whole cell lysateLane 2 : Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 50 kDaObserved band size: 50,58 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7045158 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of MCF7 cells using MBS7045158 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunoprecipitation (IP)

(Immunoprecipitating TUBB4A in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7045158 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7045158 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))

Western Blot (WB)

(Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Mouse brain tissue All lanes: TUBB4A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa)

Related Product Information for anti-TUBB4A antibody

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

NCBI and Uniprot Product Information

• NCBI GI #: 574584803
• NCBI GeneID: 10382
• NCBI Accession #: NP_001276052.1
• NCBI GenBank Nucleotide #: NM_001289123.1
• UniProt Accession #: P04350; Q969E5; B3KQP4
• NCBI Official Full Name: tubulin beta-4A chain isoform 1
• NCBI Official Synonym Full Names: tubulin beta 4A class IVa
• NCBI Official Symbol: TUBB4A
• NCBI Official Synonym Symbols: DYT4; TUBB4; beta-5
• NCBI Protein Information: tubulin beta-4A chain
• UniProt Protein Name: Tubulin beta-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB4A
• UniProt Synonym Gene Names: TUBB4; TUBB5
• UniProt Entry Name: TBB4A_HUMAN

NCBI Description

This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]

Uniprot Description

TUBB4: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; microtubule; nucleus

Molecular Function: protein binding

Biological Process: G2/M transition of mitotic cell cycle

Disease: Dystonia 4, Torsion, Autosomal Dominant