Rabbit anti-Human TUBB4A Polyclonal Antibody | anti-TUBB4A antibody

Anti-TUBB4A Antibody, Rabbit Polyclonal

Cat. Num. AAA8103211

• Gene Names: TUBB4A; DYT4; TUBB4; beta-5
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: TUBB4A; Polyclonal Antibody; Anti-TUBB4A Antibody; Rabbit Polyclonal; TUBB4A Antibody; Rabbit PAb; Antigen Affinity Purified; tubulin; beta 4A class IVa; Anti-beta-5 Antibody; Anti-DYT4 Antibody; Anti-HLD6 Antibody; Anti-TUBB4 Antibody; Anti-TUBB5 Antibody; anti-TUBB4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TUBB4A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TUBB4A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000-1:5000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human TUBB4A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TUBB4A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TUBB4A rabbit polyclonal antibody at 1:5000 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HEK293 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of TUBB4A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TUBB4A polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoskeleton.)

Related Product Information for anti-TUBB4A antibody

The tubulin beta-4A gene (TUBB4A) is associated with two different clinical conditions, dystonia type 4 (DYT4) and hypomyelination with atrophy of the basal ganglia and cerebellum (H-ABC). Dystonia-4 (DYT4) is another autosomal dominant dystonia that is characterized by onset in the second to third decade of progressive laryngeal dysphonia. DYT4 is associated with the mutation of the TUBB4A gene. DYT4-associated TUBB4A mutants themselves form aberrant tubulin networks and inhibit neuronal process growth. Mutations in TUBB4A, encoding the tubulin isoform tubulin beta class IVA (Tubb4a), result in the symptom complex of hypomyelination with atrophy of basal ganglia and cerebellum (H-ABC). Additionally, TUBB4A mutations are known to result in a broad phenotypic spectrum, ranging from primary dystonia (DYT4), isolated hypomyelination with spastic quadriplegia, and an infantile onset encephalopathy, suggesting multiple cell types may be involved.

NCBI and Uniprot Product Information

• NCBI GI #: 574584803
• NCBI GeneID: 10382
• NCBI Accession #: NP_001276052.1
• NCBI GenBank Nucleotide #: NM_001289123.1
• UniProt Accession #: P04350; Q969E5; B3KQP4
• Molecular Weight: 49,586 Da
• NCBI Official Full Name: tubulin beta-4A chain isoform 1
• NCBI Official Synonym Full Names: tubulin, beta 4A class IVa
• NCBI Official Symbol: TUBB4A
• NCBI Official Synonym Symbols: DYT4; TUBB4; beta-5
• NCBI Protein Information: tubulin beta-4A chain
• UniProt Protein Name: Tubulin beta-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB4A
• UniProt Synonym Gene Names: TUBB4; TUBB5
• UniProt Entry Name: TBB4A_HUMAN

NCBI Description

This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]

Uniprot Description

TUBB4: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: internode region of axon; microtubule; cell soma; nucleus; cytosol; myelin sheath; cilium

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; transmembrane transport; microtubule-based process

Disease: Dystonia 4, Torsion, Autosomal Dominant