TRRAP sirna

TRRAP siRNA (Human)

Cat. Num. AAA8226521

• Gene Names: TRRAP; Tra1; TR-AP; PAF400; STAF40; PAF350/400
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRRAP; TRRAP siRNA (Human); PAF400; Transformation/transcription domain-associated protein; 350/400 kDa PCAF-associated factor; PAF350/400; STAF40; Tra1 homolog; TRRAP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRRAP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3,830

• Applicable Applications for TRRAP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRRAP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRRAP sirna

siRNA to inhibit TRRAP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 347360922
• NCBI GeneID: 8295
• NCBI Accession #: NP_001231509.1
• NCBI GenBank Nucleotide #: NM_001244580.1
• UniProt Accession #: Q9Y4A5; O75218; Q9Y631; Q9Y6H4; A4D265
• Molecular Weight: 434,414 Da
• NCBI Official Full Name: transformation/transcription domain-associated protein isoform 1
• NCBI Official Synonym Full Names: transformation/transcription domain-associated protein
• NCBI Official Symbol: TRRAP
• NCBI Official Synonym Symbols: Tra1; TR-AP; PAF400; STAF40; PAF350/400
• NCBI Protein Information: transformation/transcription domain-associated protein
• UniProt Protein Name: Transformation/transcription domain-associated protein
• Protein Family: Transformation/transcription domain-associated protein
• UniProt Gene Name: TRRAP
• UniProt Synonym Gene Names: PAF400; PAF350/400
• UniProt Entry Name: TRRAP_HUMAN

NCBI Description

This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

TRRAP: Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Interacts with MYC, E2F1 and E2F4 transcription factors. Interacts directly with p53/TP53. Interacts with GCN5L2. Component of various HAT complexes. Component of the PCAF complex, at least composed of TADA2L/ADA2, SUPT3H, TADA3L/ADA3, TAF5L/PAF65-beta, TAF6L/PAF65-alpha, TAF10/TAFII30, TAF12/TAFII20, TAF9/TAFII31 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. Component of the STAGA complex, at least composed of SUPT3H, GCN5L2, SUPT7L, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Component of the BAF53 complex, at least composed of BAF53A, RUVBL1, SMARCA4/BRG1, and TRRAP, which preferentially acetylates histone H4 (and H2A) within nucleosomes. Interacts with NPAT. Interaction with TELO2 AND TTI1. Belongs to the PI3/PI4-kinase family. TRA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; Nuclear receptor co-regulator; Kinase, protein; ATYPICAL group; PIKK family; TRRAP subfamily

Chromosomal Location of Human Ortholog: 7q21.2-q22.1

Cellular Component: nucleoplasm; Golgi apparatus; PCAF complex; NuA4 histone acetyltransferase complex; nucleus

Molecular Function: protein binding; transcription coactivator activity; transcription cofactor activity; kinase activity; phosphotransferase activity, alcohol group as acceptor

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mitotic cell cycle checkpoint; histone acetylation; DNA repair; phosphorylation; histone deubiquitination