TRPM8 sirna

TRPM8 siRNA (Human)

Cat. Num. AAA8214993

• Gene Names: TRPM8; TRPP8; LTRPC6
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPM8; TRPM8 siRNA (Human); LTRPC6; TRPP8; Transient receptor potential cation channel subfamily M member 8; Long transient receptor potential channel 6; LTrpC-6; LTrpC6; Transient receptor potential p8; Trp-p8; TRPM8 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRPM8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1104

• Applicable Applications for TRPM8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPM8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPM8 sirna

siRNA to inhibit TRPM8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985.4
• NCBI GenBank Nucleotide #: NM_024080.4
• UniProt Accession #: Q7Z2W7; Q3YFM7; Q6QNH9; Q8TAC3; Q8TDX8; Q9BVK1; A0AVG2
• Molecular Weight: 79,096 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• Protein Family: TRPM8 channel-associated factor
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8
• UniProt Entry Name: TRPM8_HUMAN

Uniprot Description

TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; protein homodimerization activity; calcium channel activity

Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; response to cold; thermoception; transmembrane transport; protein homotetramerization