TRPM5 sirna

TRPM5 siRNA (Human)

Cat. Num. AAA8240636

• Gene Names: TRPM5; MTR1; LTRPC5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPM5; TRPM5 siRNA (Human); LTRPC5; MTR1; Transient receptor potential cation channel subfamily M member 5; Long transient receptor potential channel 5; LTrpC-5; LTrpC5; MLSN1- and TRP-related gene 1 protein; TRPM5 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TRPM5 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1165

• Applicable Applications for TRPM5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TRPM5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPM5 sirna

siRNA to inhibit TRPM5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 11225266
• NCBI GeneID: 29850
• NCBI Accession #: NP_055370.1
• NCBI GenBank Nucleotide #: NM_014555.3
• UniProt Accession #: Q9NZQ8; Q52LU2; Q9NY34; A6NHS0
• Molecular Weight: 97,738 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 5
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 5
• NCBI Official Symbol: TRPM5
• NCBI Official Synonym Symbols: MTR1; LTRPC5
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 5
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 5
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM5
• UniProt Synonym Gene Names: LTrpC-5; LTrpC5
• UniProt Entry Name: TRPM5_HUMAN

NCBI Description

This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. [provided by RefSeq, Oct 2010]

Uniprot Description

TRPM5: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5- triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage- dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: plasma membrane; integral to membrane

Molecular Function: potassium channel activity; ion channel activity; voltage-gated ion channel activity; sodium channel activity; calcium activated cation channel activity

Biological Process: sensory perception of taste; transmembrane transport