TRPM5 Polyclonal Antibody | anti-TRPM5 antibody

Anti-TRPM5 Antibody

Cat. Num. AAA178345

• Gene Names: TRPM5; MTR1; LTRPC5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TRPM5; Polyclonal Antibody; Anti-TRPM5 Antibody; Transient receptor potential cation channel subfamily M member 5; 9430099A16Rik; Long transient receptor potential channel 5; LTrpC-5; LTrpC5; MLSN1 and TRP related gene 1; MLSN1 and TRP related gene 1 protein; MLSN1 and TRP-related; MLSN1- and TRP-related gene 1 protein; MTR1; Novel protein similar to vertebrate transient receptor potential cation channel; subfamily M; member 5; Transient receptor potential cation channel; Trpm5; TRPM5 transient receptor potential cation channel; TRPM5_HUMAN; transient receptor potential cation channel subfamily M member 5; anti-TRPM5 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1165

• Applicable Applications for anti-TRPM5 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of TRPM5 expression in SMMC whole cell lysates (lane 1) and 293T whole cell lysates (lane 2). TRPM5 at 131KD was detected using rabbit anti- TRPM5 Antigen Affinity purified polyclonal antibody at0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(TRPM5 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti- TRPM5 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(TRPM5 was detected in paraffin-embedded sections of rat testis tissues using rabbit anti- TRPM5 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(TRPM5 was detected in paraffin-embedded sections of human prostatic cancer tissues using rabbit anti- TRPM5 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-TRPM5 antibody

Description: Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily M member 5(TRPM5) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Transient receptor potential cation channel subfamily M member 5 (TRPM5) is a protein that in humans is encoded by the TRPM5 gene. This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.

References

1. Emily R Liman (2010). "Changing Taste by Targeting the Ion Channel TRPM5".The Open Drug Discovery Journal 2: 98-102. 2. Liman ER (2007). "TRPM5 and taste transduction". Handb Exp Pharmacol. Handbook of Experimental Pharmacology 179 (179): 287-98.

NCBI and Uniprot Product Information

• NCBI GI #: 11225266
• NCBI GeneID: 29850
• NCBI Accession #: NP_055370.1
• NCBI GenBank Nucleotide #: NM_014555.3
• UniProt Accession #: Q9NZQ8; Q52LU2; Q9NY34; A6NHS0
• Molecular Weight: 97,738 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 5
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 5
• NCBI Official Symbol: TRPM5
• NCBI Official Synonym Symbols: MTR1; LTRPC5
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 5
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 5
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM5
• UniProt Synonym Gene Names: LTrpC-5; LTrpC5
• UniProt Entry Name: TRPM5_HUMAN

NCBI Description

This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca(2+) ions. It is activated by lower concentrations of intracellular Ca(2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele. [provided by RefSeq, Oct 2010]

Uniprot Description

TRPM5: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5- triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage- dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM5 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cell soma; dendrite; integral to membrane; plasma membrane

Molecular Function: calcium activated cation channel activity; ion channel activity; potassium channel activity; sodium channel activity; voltage-gated ion channel activity

Biological Process: sensory perception of taste