TPM3 sirna

TPM3 siRNA (Human)

Cat. Num. AAA8226360

• Gene Names: TPM3; TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TPM3; TPM3 siRNA (Human); Tropomyosin alpha-3 chain; Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5; TPM3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TPM3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 158

• Applicable Applications for TPM3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TPM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TPM3 sirna

siRNA to inhibit TPM3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114155144
• NCBI GeneID: 7170
• NCBI Accession #: NP_001036816.1
• NCBI GenBank Nucleotide #: NM_001043351.1
• UniProt Accession #: P06753; P12324; Q2QD06; Q5VU58; Q5VU63; Q5VU66; Q5VU71; Q5VU72; Q8TCG3; Q969Q2; Q9NQH8; D3DV71
• Molecular Weight: 18,603 Da
• NCBI Official Full Name: tropomyosin alpha-3 chain isoform Tpm3.2cy
• NCBI Official Synonym Full Names: tropomyosin 3
• NCBI Official Symbol: TPM3
• NCBI Official Synonym Symbols: TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5
• NCBI Protein Information: tropomyosin alpha-3 chain
• UniProt Protein Name: Tropomyosin alpha-3 chain
• Protein Family: Tropomyosin
• UniProt Gene Name: TPM3
• UniProt Synonym Gene Names: hTM5
• UniProt Entry Name: TPM3_HUMAN

NCBI Description

This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

TPM3: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor; Oncoprotein; Cytoskeletal; Contractile

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; growth cone; stress fiber; podosome; muscle thin filament tropomyosin; cytosol; cleavage furrow

Molecular Function: actin binding

Biological Process: muscle contraction; cell motility; muscle filament sliding

Disease: Nemaline Myopathy 1; Myopathy, Congenital, With Fiber-type Disproportion