TP53INP1 sirna

TP53INP1 siRNA (Human)

Cat. Num. AAA8214000

• Gene Names: TP53INP1; SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TP53INP1; TP53INP1 siRNA (Human); P53DINP1; SIP; Tumor protein p53-inducible nuclear protein 1; Stress-induced protein; p53-dependent damage-inducible nuclear protein 1; p53DINP1; TP53INP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TP53INP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 164

• Applicable Applications for TP53INP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TP53INP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TP53INP1 sirna

siRNA to inhibit TP53INP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209180469
• NCBI GeneID: 94241
• NCBI Accession #: NP_001129205.1
• NCBI GenBank Nucleotide #: NM_001135733.1
• UniProt Accession #: Q96A56; Q969R9; B2RCE5
• Molecular Weight: 18,244 Da
• NCBI Official Full Name: tumor protein p53-inducible nuclear protein 1 isoform b
• NCBI Official Synonym Full Names: tumor protein p53 inducible nuclear protein 1
• NCBI Official Symbol: TP53INP1
• NCBI Official Synonym Symbols: SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• NCBI Protein Information: tumor protein p53-inducible nuclear protein 1
• UniProt Protein Name: Tumor protein p53-inducible nuclear protein 1
• Protein Family: Tumor protein p53-inducible nuclear protein
• UniProt Gene Name: TP53INP1
• UniProt Synonym Gene Names: P53DINP1; SIP; p53DINP1
• UniProt Entry Name: T53I1_HUMAN

Uniprot Description

TP53INP1: Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Cell cycle regulation; Apoptosis

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: PML body; autophagic vacuole; cytoplasmic vesicle; cytosol; nucleus

Molecular Function: antioxidant activity; protein binding

Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; apoptosis; response to heat; positive regulation of transcription, DNA-dependent; autophagic cell death; response to stress; cell cycle arrest; negative regulation of cell migration; positive regulation of autophagy; autophagic vacuole formation