THEMIS sirna

THEMIS siRNA (Human)

Cat. Num. AAA8238098

• Gene Names: THEMIS; GASP; SPOT; TSEPA; C6orf190; C6orf207; bA325O24.3; bA325O24.4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: THEMIS; THEMIS siRNA (Human); C6orf190; C6orf207; Protein THEMIS; Thymocyte-expressed molecule involved in selection; THEMIS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: THEMIS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 641

• Applicable Applications for THEMIS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human THEMIS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for THEMIS sirna

siRNA to inhibit THEMIS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58218986
• NCBI GeneID: 387357
• NCBI Accession #: NP_001010923.1
• NCBI GenBank Nucleotide #: NM_001010923.2
• UniProt Accession #: Q8N1K5; Q5T3C4; Q5T3C5; Q6MZT7; A1L4F0; A8K7N1; B3KT31; B3KW32; B3KY07; F5H1J9
• Molecular Weight: 77,515 Da
• NCBI Official Full Name: protein THEMIS isoform 2
• NCBI Official Synonym Full Names: thymocyte selection associated
• NCBI Official Symbol: THEMIS
• NCBI Official Synonym Symbols: GASP; SPOT; TSEPA; C6orf190; C6orf207; bA325O24.3; bA325O24.4
• NCBI Protein Information: protein THEMIS
• UniProt Protein Name: Protein THEMIS
• Protein Family: Protein
• UniProt Gene Name: THEMIS
• UniProt Synonym Gene Names: C6orf190; C6orf207
• UniProt Entry Name: THMS1_HUMAN

NCBI Description

This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

THEMIS: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. Interacts with PLCG1, ITK and GRB2. Belongs to the themis family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: signalosome; cytoplasm; intercellular junction; nucleus

Molecular Function: protein binding

Biological Process: positive T cell selection; negative T cell selection; T cell receptor signaling pathway