TCIRG1 sirna

TCIRG1 siRNA (Human)

Cat. Num. AAA8228581

• Gene Names: TCIRG1; a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TCIRG1; TCIRG1 siRNA (Human); ATP6N1C; ATP6V0A3; V-type proton ATPase 116 kDa subunit a isoform 3; V-ATPase 116 kDa isoform a3; Osteoclastic proton pump 116 kDa subunit; OC-116 kDa; OC116; T-cell immune regulator 1; T-cell immune response cDNA7 protein; TIRC7; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3; TCIRG1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TCIRG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 830

• Applicable Applications for TCIRG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TCIRG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TCIRG1 sirna

siRNA to inhibit TCIRG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19924145
• NCBI GeneID: 10312
• NCBI Accession #: NP_006010.2
• NCBI GenBank Nucleotide #: NM_006019.3
• UniProt Accession #: Q13488; O75877; Q8WVC5
• Molecular Weight: 68,776 Da
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 3 isoform a
• NCBI Official Synonym Full Names: T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• NCBI Official Symbol: TCIRG1
• NCBI Official Synonym Symbols: a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 3
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 3
• Protein Family: V-type proton ATPase
• UniProt Gene Name: TCIRG1
• UniProt Synonym Gene Names: ATP6N1C; ATP6V0A3; V-ATPase 116 kDa isoform a3; OC-116 kDa; OC116; TIRC7
• UniProt Entry Name: VPP3_HUMAN

NCBI Description

Through alternate splicing, this gene encodes two proteins with similarity to subunits of the vacuolar ATPase (V-ATPase) but the encoded proteins seem to have different functions. V-ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TCIRG1: Part of the proton channel of V-ATPases. Seems to be directly involved in T-cell activation. Defects in TCIRG1 are the cause of osteopetrosis autosomal recessive type 1 (OPTB1); also called autosomal recessive Albers-Schonberg disease or infantile malignant osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. The features of OPTB1 are macrocephaly, progressive deafness and blindness, hepatosplenomegaly, and severe anemia beginning in early infancy or in fetal life. Deafness and blindness are generally thought to represent effects of pressure on nerves. Belongs to the V-ATPase 116 kDa subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Transporter; Transporter, ion channel; Transporter, iron; Membrane protein, multi-pass; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: phagocytic vesicle membrane; mitochondrion; lysosomal membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: vacuolar acidification; interaction with host; proton transport; ATP synthesis coupled proton transport; cellular iron ion homeostasis; positive regulation of cell proliferation; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; cellular defense response; transmembrane transport

Disease: Osteopetrosis, Autosomal Recessive 1