TBX4 sirna

TBX4 siRNA (Human)

Cat. Num. AAA8237971

• Gene Names: TBX4; SPS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBX4; TBX4 siRNA (Human); T-box transcription factor TBX4; T-box protein 4; TBX4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TBX4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 545

• Applicable Applications for TBX4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TBX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBX4 sirna

siRNA to inhibit TBX4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18129690
• NCBI GeneID: 9496
• NCBI Accession #: NP_060958.2
• NCBI GenBank Nucleotide #: NM_018488.2
• UniProt Accession #: P57082; A5PKU7; B2RMT1; B7ZLV3
• Molecular Weight: 60,275 Da
• NCBI Official Full Name: T-box transcription factor TBX4
• NCBI Official Synonym Full Names: T-box 4
• NCBI Official Symbol: TBX4
• NCBI Official Synonym Symbols: SPS
• NCBI Protein Information: T-box transcription factor TBX4
• UniProt Protein Name: T-box transcription factor TBX4
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX4
• UniProt Synonym Gene Names: T-box protein 4
• UniProt Entry Name: TBX4_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX4: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX4 are the cause of small patella syndrome (SPS); also known as ischiopatellar dysplasia or Scott-Taor syndrome. SPS is an autosomal dominant skeletal dysplasia characterized by patellar aplasia or hypoplasia and by anomalies of the pelvis and feet, including disrupted ossification of the ischia and inferior pubic rami.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; skeletal morphogenesis; multicellular organismal development; morphogenesis of an epithelium; angiogenesis; limb morphogenesis; embryonic limb morphogenesis; lung development

Disease: Small Patella Syndrome