Goat RBM20 Polyclonal Antibody | anti-RBM20 antibody

Goat anti-RBM20 (aa717-727) Antibody

Cat. Num. AAA422508

• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: RBM20; Polyclonal Antibody; Goat anti-RBM20 (aa717-727) Antibody; Goat Anti-RBM20 (aa671-681) Antibody; RNA binding motif protein 20; RNA binding motif protein 20 antibody; RBM20 antibody; RBM20 (aa717-727); anti-RBM20 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QLDKAELDERP
• Sequence Length: 1227

• Applicable Applications for anti-RBM20 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA:; antibody detection limit dilution 1:64000.; Western blot:; Approx 150kDa band observed in lysates of cell line HEK293 (calculated MW of 134kDa according to Human NP_001127835.2). Preliminary experiments also showed a 150kDa band in lU2OS cell lysate and in Human Smooth Muscle lysate. This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1?g/ml. Primary incubation 1 hour at room temperature.
• Immunogen: Peptide with sequence C-QLDKAELDERP, from the internal region of the protein sequence according to NP_001127835.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of HEK293 cell lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 197276594
• NCBI GeneID: 282996
• NCBI Accession #: NP_001127835.2
• NCBI GenBank Nucleotide #: NM_001134363.1
• NCBI Official Full Name: RNA-binding protein 20
• NCBI Official Synonym Full Names: RNA binding motif protein 20
• NCBI Official Symbol: RBM20
• NCBI Protein Information: RNA-binding protein 20
• UniProt Protein Name: RNA-binding protein 20
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM20
• UniProt Entry Name: RBM20_HUMAN

NCBI Description

This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014]

Uniprot Description

RBM20: RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin). Defects in RBM20 are the cause of cardiomyopathy dilated type 1DD (CMD1DD). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: nucleus

Molecular Function: nucleotide binding; RNA binding; zinc ion binding

Biological Process: heart development; mRNA processing; positive regulation of RNA splicing; RNA splicing

Disease: Cardiomyopathy, Dilated, 1dd