STEAP2 sirna

STEAP2 siRNA (Human)

Cat. Num. AAA8208586

• Gene Names: STEAP2; STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: STEAP2; STEAP2 siRNA (Human); PCANAP1; STAMP1; Metalloreductase STEAP2; Prostate cancer-associated protein 1; Protein up-regulated in metastatic prostate cancer; PUMPCn; Six-transmembrane epithelial antigen of prostate 2; SixTransMembrane protein of prostate 1; STEAP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: STEAP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 490

• Applicable Applications for STEAP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human STEAP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for STEAP2 sirna

siRNA to inhibit STEAP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 100913194
• NCBI GeneID: 261729
• NCBI Accession #: NP_001035755.1
• NCBI GenBank Nucleotide #: NM_001040665.1
• UniProt Accession #: Q8NFT2; Q6UXN6; Q6YPB1; Q8IUE7; A4D1F1; G5E9C6
• Molecular Weight: 47,916 Da
• NCBI Official Full Name: metalloreductase STEAP2 isoform a
• NCBI Official Synonym Full Names: STEAP family member 2, metalloreductase
• NCBI Official Symbol: STEAP2
• NCBI Official Synonym Symbols: STMP; IPCA1; PUMPCn; STAMP1; PCANAP1
• NCBI Protein Information: metalloreductase STEAP2
• UniProt Protein Name: Metalloreductase STEAP2
• Protein Family: Metalloreductase
• UniProt Gene Name: STEAP2
• UniProt Synonym Gene Names: PCANAP1; STAMP1; PUMPCn
• UniProt Entry Name: STEA2_HUMAN

NCBI Description

This gene is a member of the STEAP family and encodes a multi-pass membrane protein that localizes to the Golgi complex, the plasma membrane, and the vesicular tubular structures in the cytosol. A highly similar protein in mouse has both ferrireductase and cupric reductase activity, and stimulates the cellular uptake of both iron and copper in vitro. Increased transcriptional expression of the human gene is associated with prostate cancer progression. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

STEAP2: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Belongs to the STEAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.16.1.-; Vesicle; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q21.13

Cellular Component: trans-Golgi network transport vesicle; intracellular membrane-bound organelle; early endosome; plasma membrane; integral to Golgi membrane; endosome membrane; cytosol

Molecular Function: transporter activity; metal ion binding; cupric reductase activity

Biological Process: regulated secretory pathway; Golgi to plasma membrane transport; iron ion homeostasis; response to hormone stimulus; copper ion import; endocytosis