SPRTN sirna

SPRTN siRNA (Human)

Cat. Num. AAA8212502

• Gene Names: SPRTN; DVC1; PRO4323; spartan; C1orf124
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPRTN; SPRTN siRNA (Human); C1orf124; DVC1; SprT-like domain-containing protein Spartan; DNA damage protein targeting VCP; Protein with SprT-like domain at the N terminus; Spartan; SPRTN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPRTN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 250

• Applicable Applications for SPRTN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPRTN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPRTN sirna

siRNA to inhibit SPRTN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58331107
• NCBI GeneID: 83932
• NCBI Accession #: NP_001010984.1
• NCBI GenBank Nucleotide #: NM_001010984.3
• UniProt Accession #: Q9H040; Q5TE78; Q6UWW6; Q96BC5; Q96KA0; B1AKT0; B5MEF7
• Molecular Weight: 24,247 Da
• NCBI Official Full Name: sprT-like domain-containing protein Spartan isoform b
• NCBI Official Synonym Full Names: SprT-like N-terminal domain
• NCBI Official Symbol: SPRTN
• NCBI Official Synonym Symbols: DVC1; PRO4323; spartan; C1orf124
• NCBI Protein Information: sprT-like domain-containing protein Spartan
• UniProt Protein Name: SprT-like domain-containing protein Spartan
• UniProt Gene Name: SPRTN
• UniProt Synonym Gene Names: C1orf124; DVC1; DVC1; Spartan
• UniProt Entry Name: SPRTN_HUMAN

NCBI Description

The protein encoded by this gene may play a role in DNA repair during replication of damaged DNA. This protein recruits valosin containing protein (p97) to stalled DNA replication forks where it may prevent excessive translesional DNA synthesis and limit the number of DNA-damage induced mutations. It may also be involved in replication-related G2/M-checkpoint regulation. Deficiency of a similar protein in mouse causes chromosomal instability and progeroid phenotypes. Mutations in this gene have been associated with Ruijs-Aalfs syndrome (RJALS). Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Uniprot Description

SPRTN: Regulator of UV-induced DNA damage response: acts as a 'reader' of ubiquitinated PCNA that enhances RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. Recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA. Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis. Also required for localization of DNA polymerase eta (POLH) to sites of UV damage. Belongs to the Spartan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 1q42.12-q43

Cellular Component: nucleoplasm; nuclear speck; chromosome; nucleus

Molecular Function: ubiquitin binding; protein binding; DNA binding; metal ion binding

Biological Process: bypass DNA synthesis; positive regulation of protein ubiquitination; response to DNA damage stimulus; response to UV

Disease: Ruijs-aalfs Syndrome